Huang Qixin, Yi Wuzhou, Fan Jinghui, Chen Rui, Ma Xin, Chen Zhou, Wu Wenzi, Qian Lichun
Key Laboratory of Animal Nutrition and Feed Science in East China, Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China.
Hainan Institute of Zhejiang University, Sanya 572025, PR China.
Poult Sci. 2024 Dec;103(12):104461. doi: 10.1016/j.psj.2024.104461. Epub 2024 Oct 31.
This study investigated the effects of multi-enzyme supplementation on various aspects of laying hens, including laying performance, egg quality, intestinal health and digestive function. In total, 384 Jingfen No.6 laying hens at 65-week-age were randomly assigned to four distinct dietary treatments: a basal diet (CON), CON supplemented with 150 g/t multi-enzyme (T1), CON with 300 g/t multi-enzyme (T2), and 600 g/t multi-enzyme (T3). A significant linear (P = 0.044) and quadratic (P = 0.014) increase was observed in the laying rate, while the feed/egg ratio exhibited a linear (P = 0.001) and quadratic (P < 0.001) decrease with increasing multi-enzyme supplementation. Additionally, linear (P < 0.05) and quadratic (P < 0.05) increases were observed in yolk rate and haugh unit with increasing levels of multi-enzyme supplementation. The trypsin activity in the duodenum and crude protein digestibility showed linear (P < 0.05) and quadratic (P < 0.05) increase with the addition of multi-enzyme. Furthermore, lipase and amylase activities in the duodenum increased quadratically (P = 0.041) and linearly (P = 0.040), respectively. Both jejunal and ileal digesta viscosities showed linear (P < 0.05) and quadratic (P < 0.05) decrease with the increasing addition of multi-enzyme. Moreover, multi-enzyme supplementation significantly increased (P < 0.05) the number of goblet cells in the intestinal of the treatment groups. The mRNA expression of Occludin-1, mucin 2 (MUC-2), large neutral amino acids transporter small subunit 1 (LAT-1) in the jejunum were significantly increased (P < 0.05) in the treatment groups (T1, T2 and T3) compared to the CON group. Additionally, the mRNA expression of solute carrier family 6-member 19 (BAT-1) and large neutral amino acids transporter small subunit 4 (LAT-4) were significantly evaluated (P < 0.05) in the T2 and T3 groups, respectively. In conclusion, multi-enzyme supplementation enhanced digestive enzyme activities and intestinal barrier function, reduced intestinal digesta viscosity, and regulated mRNA expression of intestinal amino acid and lipid transporter genes, thereby improving crude protein digestibility and positively affecting laying performance and egg quality in hens.
本研究调查了添加复合酶对蛋鸡各个方面的影响，包括产蛋性能、蛋品质、肠道健康和消化功能。总共384只65周龄的京粉6号蛋鸡被随机分配到四种不同的日粮处理组：基础日粮（CON）、添加150 g/t复合酶的CON（T1）、添加300 g/t复合酶的CON（T2）和添加600 g/t复合酶的CON（T3）。随着复合酶添加量的增加，产蛋率呈显著的线性（P = 0.044）和二次曲线（P = 0.014）增加，而料蛋比呈线性（P = 0.001）和二次曲线（P < 0.001）下降。此外，随着复合酶添加量的增加，蛋黄率和哈氏单位呈线性（P < 0.05）和二次曲线（P < 0.05）增加。十二指肠中的胰蛋白酶活性和粗蛋白消化率随着复合酶的添加呈线性（P < 0.05）和二次曲线（P < 0.05）增加。此外，十二指肠中的脂肪酶和淀粉酶活性分别呈二次曲线（P = 0.041）和线性（P = 0.040）增加。空肠和回肠食糜粘度均随着复合酶添加量的增加呈线性（P < 0.05）和二次曲线（P < 0.05）下降。此外，添加复合酶显著增加了（P < 0.05）处理组鸡肠道中杯状细胞的数量。与CON组相比，处理组（T1、T2和T3）空肠中闭合蛋白-1、粘蛋白2（MUC-2）、大中性氨基酸转运体小亚基1（LAT-1）的mRNA表达显著增加（P < 0.05）。此外，溶质载体家族6成员19（BAT-1）和大中性氨基酸转运体小亚基4（LAT-4）的mRNA表达在T2组和T3组中分别得到显著评估（P < 0.05）。总之，添加复合酶可提高消化酶活性和肠道屏障功能，降低肠道食糜粘度，并调节肠道氨基酸和脂质转运体基因的mRNA表达，从而提高粗蛋白消化率，并对蛋鸡的产蛋性能和蛋品质产生积极影响。
