Rönnberg Bengt, Vapalahti Olli, Goeijenbier Marco, Reusken Chantal, Gustafsson Åke, Blomberg Jonas, Lundkvist Åke
Section of Clinical Microbiology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Infect Ecol Epidemiol. 2017 Jul 26;7(1):1350086. doi: 10.1080/20008686.2017.1350086. eCollection 2017.
: Hantaviruses are globally distributed zoonotic pathogens. Great diversity and high antigenic cross-reactivity makes diagnosis by traditional methods cumbersome. : 'Megapeptides', 119-120-mers from the amino terminus of the nucleoprotein of 16 hantaviruses, representing the four major branches of the hantavirus phylogenetic tree, were utilized in a novel IgG-based hantavirus suspension multiplex immunoassay (HSMIA) for detection of past hantavirus infections in 155 North European human samples. We compared HSMIA with established EIAs and focus reduction neutralization test (FRNT). : The Puumala hantavirus (PUUV) component in the HSMIA gave concordant results with a PUUV IgG EIA in 142 sera from Northern Sweden (of which 31 were EIA positive, 7 borderline and 104 EIA negative, sensitivity 30/31 = 97%, specificity 104/ 104 = 100%, 134/135 = 99% concordance), with another immunoassay in 40 PUUV IgG positive sera from Finland (36/40 = 90% sensitivity), and was concordant in 8 of 11 cases with PUUV and DOBV neutralization titers, respectively. Two major IgG reactivity patterns were found: (i) a PUUV-specific pattern covering phylogroup IV and its serogroups B and C; and (ii) a Dobrava virus (DOBV)-specific pattern, covering the serogroup A portion of phylogroup III. In addition, we found several minor patterns with reactivity to only one or two megapeptides indicating additional hantaviruses infecting humans in the Swedish and Finnish populations. : The broadly reactive and rational HSMIA yielded results highly correlated with the established PUUV EIAs and the NT results. It is a sensitive and specific assay, which will be suited for efficient serosurveillance of hantaviruses in humans. Its use in animals should be further investigated.
汉坦病毒是全球分布的人畜共患病原体。其高度的多样性和抗原交叉反应性使得传统方法诊断起来很麻烦。来自16种汉坦病毒核蛋白氨基末端的119 - 120肽段“巨肽”，代表汉坦病毒系统发育树的四个主要分支，被用于一种基于IgG的新型汉坦病毒悬浮多重免疫分析（HSMIA），以检测155份北欧人类样本中过去的汉坦病毒感染情况。我们将HSMIA与既定的酶免疫分析（EIA）和蚀斑减少中和试验（FRNT）进行了比较。HSMIA中的普马拉汉坦病毒（PUUV）成分与瑞典北部142份血清中的PUUV IgG EIA结果一致（其中31份EIA阳性，7份临界，104份EIA阴性，敏感性30/31 = 97%，特异性104/104 = 100%，一致性134/135 = 99%），与芬兰40份PUUV IgG阳性血清中的另一种免疫分析结果一致（敏感性36/40 = 90%），并且在11例分别针对PUUV和DOBV中和滴度的病例中有8例结果一致。发现了两种主要的IgG反应模式：（i）覆盖系统发育群IV及其血清群B和C的PUUV特异性模式；（ii）覆盖系统发育群III血清群A部分的多布拉伐病毒（DOBV）特异性模式。此外，我们发现了几种仅对一两种巨肽有反应的次要模式，表明在瑞典和芬兰人群中还有其他汉坦病毒感染人类。这种具有广泛反应性且合理的HSMIA产生的结果与既定的PUUV EIA和中和试验结果高度相关。它是一种敏感且特异的检测方法，适合于对人类汉坦病毒进行有效的血清学监测。其在动物中的应用应进一步研究。
