Department of Food and Nutritional Sciences, University of Reading, Reading, UK.
Department of Biomedical Sciences, University of Cagliari, Cagliari, Italy.
Mol Nutr Food Res. 2017 Dec;61(12). doi: 10.1002/mnfr.201700283. Epub 2017 Oct 26.
The aim of the present study was to investigate the ability of extra virgin olive oil (EVOO) polyphenols to counteract the proinflammatory effects induced by dietary and endogenous oxysterols in ex vivo immune cells.
Peripheral blood mononuclear cells (PBMCs), separated from the whole blood of healthy donors, were utilized and were stimulated with an oxysterols mixture, in the presence of physiologically relevant concentrations of the EVOO polyphenols, hydroxytyrosol, tyrosol, and homovanillic alcohol. Oxysterols significantly increased the production of proinflammatory cytokines, interleukin-1β, regulated on activation, normal T-cell expressed and secreted and macrophage migration inhibitory factor in ex vivo cultured PBMCs. Increased levels of reactive oxygen species (ROS) were also detected along with increased phosphorylation of the p38 and JNK. All phenolic compounds significantly reduced cytokine secretion induced by the oxysterols and inhibited ROS production and mitogen activated protein kinase phosphorylation.
These results suggest that extra virgin olive oil polyphenols modulate the immune response induced by dietary and endogenous cholesterol oxidation products in human immune cells and may hold benefit in controlling chronic immune and/or inflammatory processes.
本研究旨在探究特级初榨橄榄油（EVOO）多酚抵消膳食和内源性氧化固醇在体外免疫细胞中诱导的促炎作用的能力。
从健康供体的全血中分离出外周血单核细胞（PBMC），并用生理相关浓度的 EVOO 多酚、羟基酪醇、酪醇和高香草酸醇刺激，同时存在 oxysterols 混合物。Oxysterols 显著增加了体外培养的 PBMC 中促炎细胞因子白细胞介素-1β、激活调节正常 T 细胞表达和分泌因子和巨噬细胞迁移抑制因子的产生。还检测到活性氧（ROS）水平升高，同时 p38 和 JNK 的磷酸化增加。所有酚类化合物均显著降低 oxysterols 诱导的细胞因子分泌，并抑制 ROS 产生和丝裂原激活蛋白激酶磷酸化。
这些结果表明，特级初榨橄榄油多酚可调节人类免疫细胞中膳食和内源性胆固醇氧化产物引起的免疫反应，并可能有助于控制慢性免疫和/或炎症过程。
