Park Sooyeon, Kang Chul-Hyung, Won Sung-Min, Park Ji-Min, Kim Byung-Chan, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangangu, Suwon, South Korea.
University of Science and Technology (UST), 113 Gwahak-ro, Yuseong-gu, Daejeon, South Korea.
Int J Syst Evol Microbiol. 2015 Oct;65(10):3603-3608. doi: 10.1099/ijsem.0.000457.
A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, DSSK2-12T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain DSSK2-12T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain DSSK2-12T fell within the clade comprising the type strains of species of the genus Alteromonas. Strain DSSK2-12T exhibited 16S rRNA gene sequence similarity values of 97.2–98.1 % to the type strains of Alteromonas litorea, Alteromonas marina, Alteromonas hispanica and Alteromonas genovensis and of 95.39–96.98 % to those of other species of the genus Alteromonas. Strain DSSK2-12T contained Q-8 as the predominant ubiquinone and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c as the major fatty acids. The major polar lipids of strain DSSK2-12T were phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain DSSK2-12T was 48.6 mol% and its mean DNA–DNA relatedness values with the type strains of A. litorea, A. marina, A. hispanica and A. genovensis were 9–21 %. The differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain DSSK2-12T is separated from other species of the genus Alteromonas. On the basis of the data presented, strain DSSK2-12T is considered to represent a novel species of the genus Alteromonas, for which the name Alteromonas confluentis sp. nov. is proposed. The type strain is DSSK2-12T ( = KCTC 42603T = CECT 8870T).
从韩国济州岛海洋与淡水泉交汇处分离出一株革兰氏染色阴性、需氧、不形成芽孢、无鞭毛、球状、卵形或杆状的细菌菌株DSSK2-12T。菌株DSSK2-12T在30 °C、pH 7.0–8.0以及2.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树显示，菌株DSSK2-12T属于包含交替单胞菌属各物种模式菌株的进化分支。菌株DSSK2-12T与浅海交替单胞菌、海生交替单胞菌、西班牙交替单胞菌和热那亚交替单胞菌模式菌株的16S rRNA基因序列相似性值为97.2–98.1 %，与交替单胞菌属其他物种的相似性值为95.39–96.98 %。菌株DSSK2-12T以Q-8作为主要泛醌，以总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）、C16 : 0和C18 : 1ω7c作为主要脂肪酸。菌株DSSK2-12T的主要极性脂类为磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的氨基脂类。菌株DSSK2-12T的DNA G+C含量为48.6 mol%，其与浅海交替单胞菌、海生交替单胞菌、西班牙交替单胞菌和热那亚交替单胞菌模式菌株的平均DNA–DNA相关性值为9–21 %。其不同的表型特征，以及系统发育和遗传独特性表明，菌株DSSK2-12T与交替单胞菌属的其他物种不同。根据所提供的数据，菌株DSSK2-12T被认为代表交替单胞菌属的一个新物种，为此提出名为融合交替单胞菌（Alteromonas confluentis）的新名称。模式菌株为DSSK2-12T（ = KCTC 42603T = CECT 8870T）。
