Park Sooyeon, Choi Su Jung, Choi Jeehyun, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4754-4759. doi: 10.1099/ijsem.0.002370. Epub 2017 Oct 6.
A Gram-stain-negative, singly flagellated, aerobic and coccoid, ovoid or rod-shaped bacterium, designated strain JDTF-33, was isolated from a tidal flat in Jindo, an island of South Korea. Strain JDTF-33 grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, demonstrated that strain JDTF-33 belonged to the genus Paraglaciecola, joining the type strain of the species Paraglaciecola aquimarinawith 97.9 % sequence similarity. Strain JDTF-33 exhibited 16S rRNA gene sequence similarities of 97.1 and 97.0 % to the type strains of Paraglaciecola arctica and Paraglaciecola psychrophila, respectively, and of 96.1-96.6 % to the type strains of the other species of the genus Paraglaciecola. Strain JDTF-33 showed DNA-DNA relatedness values of 11-24 % to the type strains of the species P. aquimarina, P. arctica and Paraglaciecola psychrophila. Strain JDTF-33 contained Q-8 as the predominant ubiquinone and summed feature 3 (C16 : ω7c and/or C16 : 1ω6c) and C16 : 0 as the major fatty acids. The major polar lipids detected in strain JDTF-33 were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain JDTF-33 was 41.7 mol%. Differential phenotypic properties, together with phylogenetic and genetic data, demonstrate that strain JDTF-33 is separate from species of the genus Paraglaciecolawith validly published names. On the basis of the data presented, strain JDTF-33 represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola aestuariivivens sp. nov. is proposed. The type strain is JDTF-33 (=KCTC 52838=NBRC 112782).
从韩国珍岛的一处潮滩中分离出了一株革兰氏染色阴性、单鞭毛、需氧且呈球状、卵形或杆状的细菌，命名为菌株JDTF - 33。菌株JDTF - 33在30 °C、pH 7.0 - 8.0以及2.0 - 3.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株JDTF - 33属于副冰川菌属，与海副冰川菌模式菌株的序列相似性为97.9 %。菌株JDTF - 33与北极副冰川菌和嗜冷副冰川菌模式菌株的16S rRNA基因序列相似性分别为97.1 %和97.0 %，与副冰川菌属其他种模式菌株的序列相似性为96.1 - 96.6 %。菌株JDTF - 33与海副冰川菌、北极副冰川菌和嗜冷副冰川菌模式菌株的DNA - DNA相关性值为11 - 24 %。菌株JDTF - 33以Q - 8作为主要泛醌，以特征性总和3（C16 : ω7c和/或C16 : 1ω6c）以及C16 : 0作为主要脂肪酸。在菌株JDTF - 33中检测到的主要极性脂类为磷脂酰胆碱、磷脂酰甘油和磷脂酰乙醇胺。菌株JDTF - 33的DNA G + C含量为41.7 mol%。不同的表型特征，连同系统发育和遗传数据表明，菌株JDTF - 33与已有效发表名称的副冰川菌属物种不同。基于所呈现的数据，菌株JDTF - 33代表副冰川菌属的一个新物种，为此提出新名称河口副冰川菌（Paraglaciecola aestuariivivens sp. nov.）。模式菌株为JDTF - 33（=KCTC 52838=NBRC 112782）。
