Wang Zhi, Yang Xue-Dong, Zha Hai-Yan, Guo Li-Na, Shen Dan, Xu Li-Zhen, Yang Shi-Lin
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jun;42(12):2318-2322. doi: 10.19540/j.cnki.cjcmm.20170308.003.
Nine compounds, including five lignan glycosides (1-5), three sucrose esters (6-8), and one organic acid ester (9), were isolated from the ethanol extract of the roots of Securidaca inappendiculata by various chromatographic methods including silica gel, MPLC and preparative HPLC. Their structures were elucidated as acernikol-4″-O-β-D-glucopyranoside (1), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside (2), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside (3), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9'-O-β-D-glucopyranoside (4), (7R, 8S)-5-methoxydihydrodehy-drodiconiferyl alcohol 4-O-β-D-glucopyranoside (5), 3, 6'-O-diferuloylsucrose (6), 3-O-feruloyl-6'-O-sinapoylsucrose (7), sibricose A5 (8), and mehyl ferulate (9) on the basis of 1H-, 13C-NMR and MS experiments. Compounds 1-5, 8, and 9 were isolated from the Securidaca genus for the first time. Compounds 2, 3, and 7 exhibited weak cytotoxic activities against Hela and MCF-7 cell lines.
通过硅胶柱色谱、中压液相色谱（MPLC）和制备型高效液相色谱等多种色谱方法，从无柄蝉翼藤根的乙醇提取物中分离出9种化合物，包括5种木脂素糖苷（1 - 5）、3种蔗糖酯（6 - 8）和1种有机酸酯（9）。基于氢谱（1H-NMR）、碳谱（13C-NMR）和质谱（MS）实验，确定它们的结构分别为acerikol-4″-O-β-D-吡喃葡萄糖苷（1）、(7R, 8S)-二氢脱氢二松柏醇9-O-β-D-吡喃葡萄糖苷（2）、(7R, 8S)-二氢脱氢二松柏醇4-O-β-D-吡喃葡萄糖苷（3）、(7R, 8S)-二氢脱氢二松柏醇9'-O-β-D-吡喃葡萄糖苷（4）、(7R, 8S)-5-甲氧基二氢脱氢二松柏醇4-O-β-D-吡喃葡萄糖苷（5）、3, 6'-O-二阿魏酰基蔗糖（6）、3-O-阿魏酰基-6'-O-芥子酰基蔗糖（7）、sibricose A5（8）和阿魏酸甲酯（9）。化合物1 - 5、8和9首次从蝉翼藤属植物中分离得到。化合物2、3和7对Hela和MCF-7细胞系表现出较弱的细胞毒活性。
