School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research and Development, Wenhua Road 103, Shenyang, Liaoning Province, 110016, China.
J Nat Med. 2019 Jun;73(3):653-660. doi: 10.1007/s11418-019-01308-5. Epub 2019 Apr 11.
Two new compounds, namely 5-hydroxy-7-methoxy-6-methylchromone (1), and sesquiterpene X (6), together with 21 known compounds were isolated from the twigs and leaves of Cephalotaxus fortunei Hook. f. The structures of 1-23 were elucidated on the basis of spectroscopic analysis (1D/2D NMR, HR-ESI-MS and IR) and comparison with literature. The absolute configuration of compound 6 was determined by means of electronic circular dichroism calculation. The in vitro anti-inflammatory activities of all compounds were assayed in RAW 264.7 cells by assessing lipopolysaccharide-induced nitric oxide production. Compounds 1 and 6 exhibited weak effects with percentage inhibitions of 24% and 35.60%, respectively. In addition, compounds 4, 9, and 14 have the potential to be developed as therapeutic agents for inflammatory diseases because of their significant anti-inflammatory activities and high content in C. fortunei.
从三尖杉的枝叶中分离得到了两个新化合物，分别为 5-羟基-7-甲氧基-6-甲基色酮（1）和倍半萜 X（6），以及 21 个已知化合物。根据光谱分析（1D/2D NMR、HR-ESI-MS 和 IR）和文献比较，确定了 1-23 的结构。通过电子圆二色性计算确定了化合物 6 的绝对构型。通过评估脂多糖诱导的一氧化氮产生，在 RAW 264.7 细胞中测试了所有化合物的体外抗炎活性。化合物 1 和 6 的抑制率分别为 24%和 35.60%，具有较弱的抗炎作用。此外，由于其显著的抗炎活性和在三尖杉中的高含量，化合物 4、9 和 14 具有开发为治疗炎症性疾病的药物的潜力。
