National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box No.577, Jhang Road, Faisalabad, Pakistan; Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan.
Faculty of Chemistry, Department of Physical Chemistry, Waehringerstrasse 38, 1090, University of Vienna, Austria.
J Hazard Mater. 2018 Jan 15;342:96-106. doi: 10.1016/j.jhazmat.2017.08.014. Epub 2017 Aug 8.
Nanotechnology holds great promise for the fabrication of versatile materials that can be used as sensor platforms for the highly selective detection of analytes. In this research article we report a new nanohybrid material, where 3D imprinted nanostructures are constructed. First, copper nanoparticles are deposited on carbon nanotubes and then a hybrid structure is formed by coating molecularly imprinted polymer on 3D CNTs@Cu NPs; and a layer by layer assembly is achieved. SEM and AFM revealed the presence of Cu NPs (100-500nm) anchored along the whole length of CNTs, topped with imprinted layer. This material was applied to fabricate an electrochemical sensor to monitor a model veterinary drug, chloramphenicol. The high electron transfer ability and conductivity of the prepared material produced sensitive response, whereas, molecular imprinting produces selectivity towards drug detection. The sensor responses were found concentration dependent and the detection limit was calculated to be 10μM (S/N=3). Finally, we showed how changing the polymer composition, the extent of cross linking, and sensor layer thickness greatly affects the number of binding sites for the recognition of drug. This work paves the way to build variants of 3D imprinted materials for the detection of other kinds of biomolecules and antibiotics.
纳米技术在制造多功能材料方面具有巨大的应用前景，这些材料可以作为传感器平台，用于高度选择性地检测分析物。在本研究论文中，我们报告了一种新的纳米杂化材料，其中构建了 3D 压印纳米结构。首先，将铜纳米粒子沉积在碳纳米管上，然后通过在 3D CNTs@Cu NPs 上涂覆分子印迹聚合物形成杂化结构；并实现了层层组装。SEM 和 AFM 揭示了存在沿着 CNTs 全长锚定的 Cu NPs（100-500nm），顶部覆盖有印迹层。该材料被应用于制造电化学传感器来监测模型兽用药物氯霉素。所制备材料的高电子转移能力和导电性产生了灵敏的响应，而分子印迹则对药物检测产生了选择性。传感器响应被发现与浓度有关，检测限计算为 10μM（S/N=3）。最后，我们展示了如何改变聚合物组成、交联程度和传感器层厚度，从而极大地影响用于识别药物的结合位点数量。这项工作为构建用于检测其他种类的生物分子和抗生素的 3D 印迹材料变体铺平了道路。
