Prelich G, Kostura M, Marshak D R, Mathews M B, Stillman B
Nature. 1987;326(6112):471-5. doi: 10.1038/326471a0.
Cell-free extracts prepared from human 293 cells, supplemented with purified SV40 large-T antigen, support replication of plasmids containing the SV40 origin of DNA replication. A cellular protein (Mr approximately 36,000) that is required for efficient SV40 DNA synthesis in vitro has been purified from these extracts. This protein is recognized by human autoantibodies and is identified as the cell-cycle regulated protein known as proliferating cell nuclear antigen (PCNA) or cyclin.
从人293细胞制备的无细胞提取物，添加纯化的猿猴病毒40大T抗原后，可支持含有猿猴病毒40 DNA复制起点的质粒的复制。一种体外高效猿猴病毒40 DNA合成所需的细胞蛋白（分子量约36,000）已从这些提取物中纯化出来。这种蛋白可被人自身抗体识别，并被鉴定为细胞周期调节蛋白，即增殖细胞核抗原（PCNA）或细胞周期蛋白。
