使用ISHAGE方案对造血干细胞进行计数的单平台与双平台分析
Single Versus Dual Platform Analysis for Hematopoietic Stem Cell Enumeration Using ISHAGE Protocol.
作者信息
Naithani Rahul, Dayal Nitin, Dixit Gaurav
机构信息
Division of Hematology and Bone Marrow Transplantation, Max Superspeciality Hospital, New Delhi, India.
Department of Lab Medicine, Max Superspeciality Hospital, New Delhi, India.
出版信息
Indian J Hematol Blood Transfus. 2017 Sep;33(3):370-374. doi: 10.1007/s12288-016-0749-9. Epub 2016 Dec 1.
Abstract
Hematopoietic stem cell transplantation is curative therapy in benign and malignant diseases. Adequate stem cell dose is one of the most important marker of engraftment. Several methods have been developed to enumerate CD34+ cells. We present our data on 147 samples analysis. There was a clear linear correlation between two methods. Both methods were effective. Both single vs dual platform analysis yield similar results. Single platform analysis is easier to perform. In terms of cost reduction dual platform analysis is better.
摘要
造血干细胞移植是治疗良性和恶性疾病的有效疗法。足够的干细胞剂量是植入的最重要标志之一。已经开发了几种方法来计数CD34+细胞。我们展示了对147个样本分析的数据。两种方法之间存在明显的线性相关性。两种方法都有效。单平台分析与双平台分析产生的结果相似。单平台分析更容易操作。在降低成本方面，双平台分析更好。
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