• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用ISHAGE方案对造血干细胞进行计数的单平台与双平台分析

Single Versus Dual Platform Analysis for Hematopoietic Stem Cell Enumeration Using ISHAGE Protocol.

作者信息

Naithani Rahul, Dayal Nitin, Dixit Gaurav

机构信息

Division of Hematology and Bone Marrow Transplantation, Max Superspeciality Hospital, New Delhi, India.

Department of Lab Medicine, Max Superspeciality Hospital, New Delhi, India.

出版信息

Indian J Hematol Blood Transfus. 2017 Sep;33(3):370-374. doi: 10.1007/s12288-016-0749-9. Epub 2016 Dec 1.

DOI:10.1007/s12288-016-0749-9
PMID:28824239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5544640/
Abstract

Hematopoietic stem cell transplantation is curative therapy in benign and malignant diseases. Adequate stem cell dose is one of the most important marker of engraftment. Several methods have been developed to enumerate CD34+ cells. We present our data on 147 samples analysis. There was a clear linear correlation between two methods. Both methods were effective. Both single vs dual platform analysis yield similar results. Single platform analysis is easier to perform. In terms of cost reduction dual platform analysis is better.

摘要

造血干细胞移植是治疗良性和恶性疾病的有效疗法。足够的干细胞剂量是植入的最重要标志之一。已经开发了几种方法来计数CD34+细胞。我们展示了对147个样本分析的数据。两种方法之间存在明显的线性相关性。两种方法都有效。单平台分析与双平台分析产生的结果相似。单平台分析更容易操作。在降低成本方面，双平台分析更好。

相似文献

1
Single Versus Dual Platform Analysis for Hematopoietic Stem Cell Enumeration Using ISHAGE Protocol.使用ISHAGE方案对造血干细胞进行计数的单平台与双平台分析
Indian J Hematol Blood Transfus. 2017 Sep;33(3):370-374. doi: 10.1007/s12288-016-0749-9. Epub 2016 Dec 1.
2
Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells in leukapheresis product and bone marrow for clinical transplantation: a comparison of three methods.流式细胞术对临床移植用白细胞单采产品和骨髓中CD34+造血干细胞和祖细胞的计数：三种方法的比较
Folia Histochem Cytobiol. 2006;44(1):53-60.
3
Flow cytometric enumeration of CD34+ hematopoietic stem cells: A comparison between single- versus dual-platform methodology using the International Society of Hematotherapy and Graft Engineering protocol.CD34+造血干细胞的流式细胞术计数：采用国际血液治疗与移植工程学会方案对单平台与双平台方法的比较
Asian J Transfus Sci. 2019 Jan-Jun;13(1):43-46. doi: 10.4103/ajts.AJTS_83_18.
4
Validation of the single-platform ISHAGE method for CD34(+) hematopoietic stem and progenitor cell enumeration in an international multicenter study.单平台ISHAGE法用于CD34(+)造血干细胞和祖细胞计数的国际多中心研究验证
Cytotherapy. 2003;5(1):55-65. doi: 10.1080/14653240310000083.
5
Validation of the single-platform ISHAGE protocol for enumeration of CD34+ hematopoietic stem cells in umbilical cord blood in a Brazilian center.单平台ISHAGE方案在巴西某中心用于脐带血中CD34+造血干细胞计数的验证
Hematol Transfus Cell Ther. 2022 Jan-Mar;44(1):49-55. doi: 10.1016/j.htct.2020.09.151. Epub 2020 Dec 4.
6
Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers.基于ISHAGE的两种单平台CD34计数方案在BD FACSCalibur和FACSCanto流式细胞仪上的比较。
Cytotherapy. 2009;11(5):595-605. doi: 10.1080/14653240902923161.
7
Enumeration of CD34+ cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy.脐血中CD34+细胞的计数：基于ISHAGE设门策略的单平台流式细胞术方法的一种变体
Cytometry. 2001 Aug 15;46(4):254-61. doi: 10.1002/cyto.1136.
8
Comparison of single and dual platform methodologies for the estimation of CD34+ hematopoietic progenitor cells: correlation with colony assay.用于估算CD34+造血祖细胞的单平台和双平台方法的比较：与集落测定的相关性
Int J Biol Markers. 2002 Oct-Dec;17(4):259-67. doi: 10.5301/jbm.2008.5044.
9
Comparison of three methods of CD34+ cell enumeration in peripheral blood: dual-platform ISHAGE protocol versus single-platform, versus microvolume fluorimetry.
Cytotherapy. 2000;2(5):371-6. doi: 10.1080/146532400539279.
10
ISHAGE protocol: are we doing it correctly?ISHAGE 方案：我们是否正确执行？
Cytometry B Clin Cytom. 2012 Jan;82(1):9-17. doi: 10.1002/cyto.b.20612. Epub 2011 Sep 13.

引用本文的文献

1
Innovator Filgrastim versus Generic Filgrastim in Hematopoietic Stem Cell Transplantation Mobilization.创新型非格司亭与非格司亭仿制药在造血干细胞移植动员中的比较
South Asian J Cancer. 2021 Sep 4;10(3):172-174. doi: 10.1055/s-0041-1729446. eCollection 2021 Sep.
2
Tandem Autologous Hematopoietic Stem Cell Transplantation in Very Young Patients with Multiple Myeloma.极年轻多发性骨髓瘤患者的串联自体造血干细胞移植
South Asian J Cancer. 2020 Oct;9(4):233-235. doi: 10.1055/s-0041-1727068. Epub 2021 Jun 14.
3
Establishing the hematopoietic stem cell transplant (HSCT) in a developing country; the journey of HSCT in Semarang, Indonesia.在一个发展中国家建立造血干细胞移植（HSCT）；印度尼西亚三宝垄的HSCT历程。
Bone Marrow Transplant. 2021 Jan;56(1):270-273. doi: 10.1038/s41409-020-0973-7. Epub 2020 Jun 17.
4
Flow cytometric enumeration of CD34+ hematopoietic stem cells: A comparison between single- versus dual-platform methodology using the International Society of Hematotherapy and Graft Engineering protocol.CD34+造血干细胞的流式细胞术计数：采用国际血液治疗与移植工程学会方案对单平台与双平台方法的比较
Asian J Transfus Sci. 2019 Jan-Jun;13(1):43-46. doi: 10.4103/ajts.AJTS_83_18.
5
Highlights of Abstracts on Hematopoietic Stem Cell Transplant in Annual Conference of ISHBT 2018.2018年国际造血干细胞移植学会年会造血干细胞移植摘要亮点
Indian J Hematol Blood Transfus. 2019 Jan;35(1):12-16. doi: 10.1007/s12288-018-01067-5. Epub 2019 Jan 14.
6
Autologous Hematopoietic Stem Cell Transplantation for Multiple Myeloma in India.印度自体造血干细胞移植治疗多发性骨髓瘤
Indian J Hematol Blood Transfus. 2018 Jul;34(3):564-565. doi: 10.1007/s12288-018-0951-z. Epub 2018 Mar 22.

本文引用的文献

1
CD34+ cell enumeration by flow cytometry: a comparison of systems and methodologies.流式细胞术检测 CD34+ 细胞：系统与方法的比较。
Arch Pathol Lab Med. 2011 Jul;135(7):909-14. doi: 10.5858/2010-0119-0AR.1.
2
Number of viable CD34(+) cells reinfused predicts engraftment in autologous hematopoietic stem cell transplantation.回输的活CD34(+)细胞数量可预测自体造血干细胞移植中的植入情况。
Bone Marrow Transplant. 2002 Jun;29(12):967-72. doi: 10.1038/sj.bmt.1703575.
3
Comparison of three methods of CD34+ cell enumeration in peripheral blood: dual-platform ISHAGE protocol versus single-platform, versus microvolume fluorimetry.
Cytotherapy. 2000;2(5):371-6. doi: 10.1080/146532400539279.
4
Single- versus dual-platform assays for human CD34+ cell enumeration.用于人CD34+细胞计数的单平台与双平台检测法
Cytometry. 1999 Dec 15;38(6):274-9.
5
Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis.CD34+造血干细胞和祖细胞的流式细胞术计数。欧洲临床细胞分析工作组。
Cytometry. 1998 Jun 15;34(3):128-42. doi: 10.1002/(sici)1097-0320(19980615)34:3<128::aid-cyto3>3.0.co;2-d.
6
Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. International Society of Hematotherapy and Graft Engineering.基于ISHAGE指南的单平台流式细胞术绝对CD34+细胞计数。国际血液治疗与移植工程学会。
Cytometry. 1998 Apr 15;34(2):61-70.
7
Quality assurance of stem cell enumeration by flow cytometry. Canadian QASE Study Group.流式细胞术进行干细胞计数的质量保证。加拿大质量保证研究组。
Cytometry. 1997 Dec 15;30(6):296-303. doi: 10.1002/(sici)1097-0320(19971215)30:6<296::aid-cyto5>3.0.co;2-e.
8
The influence of flow cytometric gating strategy on the standardization of CD34+ cell quantitation: an Australian multicenter study. Australasian BMT Scientists Study Group.流式细胞术设门策略对CD34+细胞定量标准化的影响：一项澳大利亚多中心研究。澳大拉西亚骨髓移植科学家研究小组
J Hematother. 1996 Dec;5(6):605-16. doi: 10.1089/scd.1.1996.5.605.
9
Performance evaluation of the FACSCount System: a dedicated system for clinical cellular analysis.FACSCount系统的性能评估：一种用于临床细胞分析的专用系统。
Cytometry. 1996 Mar 15;26(1):52-9. doi: 10.1002/(SICI)1097-0320(19960315)26:1<52::AID-CYTO8>3.0.CO;2-I.
10
The composition of CD34 subpopulations differs between bone marrow, blood and cord blood.CD34亚群的组成在骨髓、血液和脐带血之间存在差异。
Bone Marrow Transplant. 1996 Feb;17(2):169-78.