Soares Rogério Nogueira, Reimer Raylene A, Doyle-Baker Patricia K, Murias Juan M
1 Faculty of Kinesiology, University of Calgary, Calgary, AB, Canada.
2 Department of Biochemistry & Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Diab Vasc Dis Res. 2017 Nov;14(6):502-509. doi: 10.1177/1479164117725478. Epub 2017 Aug 21.
To non-invasively evaluate differences in oxidative metabolism in individuals with obesity compared to normal weight using the near-infrared spectroscopy and vascular occlusion technique during hyperglycaemia.
In all, 16 normal-weight individuals (body mass index: 21.3 ± 1.7 kg/m) and 13 individuals with obesity (body mass index: 34.4 ± 2.0 kg/m) had five vascular occlusion tests (pre, 30, 60, 90 and 120 min after glucose ingestion). Oxygen utilization was estimated from the area under the curve of the deoxyhemoglobin [HHb] signal during occlusion. Muscle reperfusion was derived from the area above the curve after cuff release.
The deoxyhemoglobin area under the curve during occlusion of the normal-weight individuals increased from 15,732 ± 2344 (% . s) at pre to 18,930 ± 3226 (% . s) ( p < 0.05) at 90 min after glucose ingestion. The deoxyhemoglobin area under the curve during occlusion decreased significantly from 14,695 ± 3341 (% . s) at pre to 11,273 ± 1825 (% . s) ( p < 0.05) and 11,360 ± 1750 (% . s) ( p < 0.05) at 30 and 60 min, respectively, after glucose ingestion. The area above the curve of deoxyhemoglobin during reperfusion decreased significantly from 6450 ± 765 (% . s) at pre to 4830 ± 963 (% . s) ( p < 0.05) at 60 min and to 4210 ± 595 (% . s) ( p < 0.01) at 90 min in normal-weight individuals after glucose ingestion, with no changes observed in individuals with obesity.
This study confirmed in vivo and non-invasively the metabolic inflexibility of skeletal muscle in individuals with obesity during hyperglycaemia.
利用近红外光谱和血管阻断技术，在高血糖期间非侵入性评估肥胖个体与正常体重个体氧化代谢的差异。
总共16名正常体重个体（体重指数：21.3±1.7kg/m²）和13名肥胖个体（体重指数：34.4±2.0kg/m²）进行了五次血管阻断试验（葡萄糖摄入前、摄入后30、60、90和120分钟）。通过阻断期间脱氧血红蛋白[HHb]信号曲线下面积估算氧利用率。肌肉再灌注由袖带松开后曲线以上的面积得出。
正常体重个体阻断期间脱氧血红蛋白曲线下面积从摄入前的15732±2344（%·s）增加至葡萄糖摄入后90分钟的18930±3226（%·s）（p<0.05）。肥胖个体阻断期间脱氧血红蛋白曲线下面积在葡萄糖摄入后30分钟和60分钟分别从摄入前的14695±3341（%·s）显著降至11273±1825（%·s）（p<0.05）和11360±1750（%·s）（p<0.05）。正常体重个体葡萄糖摄入后再灌注期间脱氧血红蛋白曲线以上面积从摄入前的6450±765（%·s）在60分钟时显著降至4830±963（%·s）（p<0.05），在90分钟时降至4210±595（%·s）（p<0.01），肥胖个体未观察到变化。
本研究在体内非侵入性地证实了肥胖个体在高血糖期间骨骼肌的代谢不灵活性。
