补骨脂果实中的异黄酮和抗炎成分。

Isoflavones and anti-inflammatory constituents from the fruits of Psoralea corylifolia.

作者信息

Chen Chiang-Hsiang, Hwang Tsong-Long, Chen Li-Chai, Chang Tsung-Hsien, Wei Chun-Sheng, Chen Jih-Jung

机构信息

Faculty of Pharmacy, School of Pharmaceutical Sciences, National Yang-Ming University, Taipei, 112, Taiwan; Department of Pharmacy, Tajen University, Pingtung, 907, Taiwan.

Graduate Institute of Natural Products, College of Medicine, Chang Gung University, Taoyuan, 333, Taiwan; Research Center for Industry of Human Ecology, Research Center for Chinese Herbal Medicine, Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, 333, Taiwan; Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, 333, Taiwan.

出版信息

Phytochemistry. 2017 Nov;143:186-193. doi: 10.1016/j.phytochem.2017.08.004. Epub 2017 Aug 18.

DOI:10.1016/j.phytochem.2017.08.004

PMID:28825980

Abstract

The fruits of Psoralea corylifolia, known as Psoraleae Fructus (Buguzhi in Chinese), are traditionally used for the treatment of spermatorrhea, nephritis, asthma, pollakiuria, and various inflammatory diseases. Three previously undescribed isoflavone derivatives, 7-O-methylcorylifol A, 7-O-isoprenylcorylifol A, and 7-O-isoprenylneobavaisoflavone, have been isolated from the fruits of P. corylifolia, together with 9 known compounds. The structures of these compounds were determined through spectroscopic and MS analyses. Among the isolated compounds, 7-O-methylcorylifol A and psoralen exhibited potent inhibition (IC values ≤ 10.89 μM) of superoxide anion generation by human neutrophils in response to N-formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B (fMLP/CB). 7-O-Isoprenylcorylifol A, 7-O-isoprenylneobavaisoflavone, and 12,13-dihydro-12,13-epoxybakuchiol inhibited fMLP/CB-induced elastase release with IC values ≤ 14.30 μM. In addition, 7-O-isoprenylcorylifol A, bakuchiol, 12,13-dihydro-12,13-epoxybakuchiol, and psoralidin showed potent inhibition with IC values ≤ 36.65 μM, against lipopolysaccharide (LPS)-induced nitric oxide (NO) generation.

摘要

补骨脂的果实，即补骨脂（中文名为补骨脂），传统上用于治疗遗精、肾炎、哮喘、尿频以及各种炎症性疾病。从补骨脂果实中分离出了3种此前未被描述的异黄酮衍生物，即7-O-甲基补骨脂酚A、7-O-异戊烯基补骨脂酚A和7-O-异戊烯基新补骨脂异黄酮，以及9种已知化合物。通过光谱分析和质谱分析确定了这些化合物的结构。在分离出的化合物中，7-O-甲基补骨脂酚A和补骨脂素对人中性粒细胞因N-甲酰-L-蛋氨酰-L-亮氨酰-L-苯丙氨酸/细胞松弛素B（fMLP/CB）刺激而产生超氧阴离子具有显著抑制作用（IC值≤10.89 μM）。7-O-异戊烯基补骨脂酚A、7-O-异戊烯基新补骨脂异黄酮和12,13-二氢-12,13-环氧补骨脂酚对fMLP/CB诱导的弹性蛋白酶释放具有抑制作用，IC值≤14.30 μM。此外，7-O-异戊烯基补骨脂酚A、补骨脂酚、12,13-二氢-12,13-环氧补骨脂酚和补骨脂定对脂多糖（LPS）诱导的一氧化氮（NO）生成具有显著抑制作用，IC值≤36.65 μM。