Lee Tzong-Huei, Chen Yuan-Chih, Hwang Tsong-Long, Shu Chih-Wen, Sung Ping-Jyun, Lim Yun-Ping, Kuo Wen-Lung, Chen Jih-Jung
Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 110, Taiwan.
Department of Pharmacy & Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung 907, Taiwan.
Int J Mol Sci. 2014 May 28;15(6):9566-78. doi: 10.3390/ijms15069566.
The fruit of Cnidium monnieri is commercially used as healthcare products for the improvement of impotence and skin diseases. Three new coumarins, 3'-O-methylmurraol (1), rel-(1'S,2'S)-1'-O-methylphlojodicarpin (2), and (1'S,2'S)-1'-O-methylvaginol (3), have been isolated from the fruits of C. monnieri, together with 14 known compounds (4-17). The structures of these new compounds were determined through spectroscopic and MS analyses. Compounds 1, 4-12, and 14-17 exhibited inhibition (IC50 ≤ 7.31 µg/mL) of superoxide anion generation by human neutrophils in response to formyl-l-methionyl-l-leucyl-l-phenylalanine/cytochalasin B (fMLP/CB). Compounds 7, 9-11, 15, and 17 inhibited fMLP/CB-induced elastase release with IC50 values ≤7.83 µg/mL. This investigation reveals that bioactive isolates (especially 6, 7, 14, and 17) could be further developed as potential candidates for the treatment or prevention of various inflammatory diseases.
蛇床子的果实作为改善阳痿和皮肤病的保健品在商业上有应用。从蛇床子果实中分离出了三种新的香豆素，即3'-O-甲基莫雷醇（1）、反式-(1'S,2'S)-1'-O-甲基弗洛乔迪卡品（2）和(1'S,2'S)-1'-O-甲基阴道醇（3），以及14种已知化合物（4 - 17）。这些新化合物的结构通过光谱和质谱分析确定。化合物1、4 - 12以及14 - 17对人中性粒细胞因甲酰基-L-蛋氨酰-L-亮氨酰-L-苯丙氨酸/细胞松弛素B（fMLP/CB）刺激产生超氧阴离子具有抑制作用（IC50≤7.31μg/mL）。化合物7、9 - 11、15和17以IC50值≤7.83μg/mL抑制fMLP/CB诱导的弹性蛋白酶释放。该研究表明，具有生物活性的分离物（尤其是6、7、14和17）可进一步开发成为治疗或预防各种炎症性疾病的潜在候选药物。
