Kristiansen K, Baloda S B, Larsen J L, Wadström T
Acta Pathol Microbiol Immunol Scand B. 1987 Feb;95(1):57-63. doi: 10.1111/j.1699-0463.1987.tb03087.x.
Fifty Salmonella dublin strains isolated from cattle and human diarrhoeal cases were assayed for toxin production, haemagglutination, cell-surface hydrophobicity and fibronectin-binding properties. Most strains (65% of tested) produced cytotonic toxins and cytotoxic factors when tested on Chinese hamster ovary (CHO) cells and rabbit skin test. However, only three strains produced a skin-permeability factor as determined in pig skin intra-dermal tests. None of the strains were positive in pig intestinal loop tests. Six of the 32 strains tested for 125I-fibronectin and its 125I-29 kDa N-terminal domain binding showed 10-17% and 6-10% binding, respectively. Most of the strains expressed mannose-sensitive haemagglutination (MSHA) (76%) and high cell-surface hydrophobicity (74%) when grown at 37 degrees C. At 20 degrees C the expression of MSHA and especially the expression of high cell-surface hydrophobicity were reduced. Twelve strains grown at 37 degrees C did not haemagglutinate erythrocytes from five animal species used in this study, while six of these strains expressed high cell-surface hydrophobicity. Salmonella dublin strains isolated in Denmark appeared to express a higher frequency of fimbriae type 1 (MSHA) and a lower frequency of high cell-surface hydrophobicity than the strains from external sources.
对从牛和人类腹泻病例中分离出的50株都柏林沙门氏菌菌株进行了毒素产生、血凝、细胞表面疏水性和纤连蛋白结合特性的检测。在对中国仓鼠卵巢（CHO）细胞和兔皮肤试验中进行检测时，大多数菌株（65%的受试菌株）产生了细胞紧张毒素和细胞毒性因子。然而，在猪皮肤皮内试验中，只有三株菌株产生了皮肤通透性因子。在猪肠袢试验中，所有菌株均为阴性。在检测125I-纤连蛋白及其125I-29 kDa N端结构域结合的32株菌株中，有6株分别显示出10%-17%和6%-10%的结合率。大多数菌株在37℃生长时表现出甘露糖敏感血凝（MSHA）（76%）和高细胞表面疏水性（74%）。在20℃时，MSHA的表达，尤其是高细胞表面疏水性的表达降低。在37℃生长的12株菌株不能凝集本研究中使用的五种动物的红细胞，而其中6株菌株表现出高细胞表面疏水性。与来自外部来源的菌株相比，在丹麦分离的都柏林沙门氏菌菌株似乎表达1型菌毛（MSHA）的频率更高，而高细胞表面疏水性的频率更低。
