Kuo Winston Patrick, Tigges John C, Toxavidis Vasilis, Ghiran Ionita
CloudHealth Genomics, Ltd, Shanghai, China.
Weschester Biotech Project, Asbury Park, NJ, USA.
Methods Mol Biol. 2017;1660:15-22. doi: 10.1007/978-1-4939-7253-1_2.
During their lifetime, like all other cell types, red blood cells (RBCs) release both exosomes and plasma membrane derived EVs (ectosomes). RBC exosomes are formed only during the development of RBCs in bone marrow, and are released following the fusion of microvesicular bodies (MVB) with the plasma membrane. On the other hand, RBC EVs are generated during normal aging of RBCs in circulation by budding of the plasma membrane due to complement -mediated calcium influx, followed by vesicle shedding. This makes red blood cells and stored red cells a reliable source of EVs for basic and clinical research.
在其生命周期中，与所有其他细胞类型一样，红细胞（RBCs）会释放外泌体和源自质膜的细胞外囊泡（ectosomes）。红细胞外泌体仅在骨髓中红细胞发育过程中形成，并在微囊泡体（MVB）与质膜融合后释放。另一方面，循环中的红细胞在正常衰老过程中，由于补体介导的钙内流导致质膜出芽，随后囊泡脱落，从而产生红细胞细胞外囊泡。这使得红细胞和储存的红细胞成为基础研究和临床研究中可靠的细胞外囊泡来源。
