Faculty of Pharmacy, Pharmaceutical Development of Green Innovations Group (PDGIG), Silpakorn University, Nakhon Pathom, 73000, Thailand.
Faculty of Science, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Ramkhamhaeng University, Bangkok, Thailand.
AAPS PharmSciTech. 2018 Jan;19(1):481-488. doi: 10.1208/s12249-017-0855-5. Epub 2017 Aug 21.
The purpose of the present study was to evaluate the use of cationic niosomes composed of Span20:cholesterol:cationic lipid (N ,N -dimyristeroyloxyethyl-spermine) at the molar ratio of 2.5:2.5:0.5 mM combined with hollow microneedle (MN) devices for in vivo skin immunization of plasmid DNA-encoding ovalbumin (pOVA). The results revealed that using hollow MNs with cationic niosomes for pOVA penetration successfully induced both humoral and cell-mediated immune responses including immunoglobulin G (IgG) antibody responses, interleukin-4 (IL-4), and interferon gamma (IFN-γ) cytokine secretion. When using hollow MNs with cationic niosome/pOVA complexes, the immune response was superior to naked pOVA, which testifies the increased amount of IgG antibody responses and cytokine secretion. In comparison with conventional subcutaneous (SC) injections, using hollow MNs with cationic niosome/pOVA complexes induced a higher level of both IgG immune response and cytokine release. Moreover, a group of mice immunized with hollow MNs did not show infection or bleeding on the skin. Consequently, targeted delivery of pOVA using cationic niosomes combined with hollow MNs might prove a promising vaccination method for skin vaccination.
本研究旨在评估由 2.5:2.5:0.5 mM 摩尔比的 Span20:胆固醇:阳离子脂质（N, N-二肉豆蔻酰氧基乙基- spermine）组成的阳离子脂质体与中空微针（MN）装置联合用于体内皮肤免疫质粒 DNA 编码卵清蛋白（pOVA）的应用。结果表明，使用带有阳离子脂质体的中空 MN 进行 pOVA 透皮处理可成功诱导体液和细胞介导的免疫应答，包括免疫球蛋白 G（IgG）抗体应答、白细胞介素-4（IL-4）和干扰素-γ（IFN-γ）细胞因子的分泌。当使用带有阳离子脂质体/pOVA 复合物的中空 MN 时，免疫应答优于裸 pOVA，这证明 IgG 抗体应答和细胞因子分泌的量增加。与传统的皮下（SC）注射相比，使用带有阳离子脂质体/pOVA 复合物的中空 MN 可诱导更高水平的 IgG 免疫应答和细胞因子释放。此外，一组用中空 MN 免疫的小鼠皮肤未出现感染或出血。因此，使用阳离子脂质体联合中空 MN 进行靶向 pOVA 给药可能被证明是一种有前途的皮肤疫苗接种方法。
