使用体内灌注系统研究鸡空肠和盲肠对3-氧甲基-D-葡萄糖的吸收

Jejunal and cecal 3-oxy-methyl-D-glucose absorption in chicken using a perfusion system in vivo.

作者信息

Vinardell M P, Lopera M T

出版信息

Comp Biochem Physiol A Comp Physiol. 1987;86(4):625-7. doi: 10.1016/0300-9629(87)90612-8.

Abstract

3-oxy-methyl-D-glucose (3-OMG) absorption by jejunum and caecum has been studied in the domestic fowl in vivo, with luminal perfusion, during 5 min periods. The diffusion component was evaluated in the presence of phloridzin (10(-3) M) that inhibits the active transport mechanism. Kd of jejunal and cecal diffusion of the monosaccharide have been calculated, showing a similar value. The Kt and Vmax of 3-OMG absorption were calculated using a graphical method for the two intestinal segments. The caecum showed a lower Kt and Vmax than the jejunum did.

摘要

在家禽体内，通过肠腔灌注的方法，在5分钟时间段内研究了空肠和盲肠对3-氧甲基-D-葡萄糖（3-OMG）的吸收情况。在存在根皮苷（10⁻³ M）的情况下评估扩散成分，根皮苷可抑制主动转运机制。已计算出单糖在空肠和盲肠扩散的解离常数（Kd），结果显示二者数值相似。使用图形法计算了两个肠段3-OMG吸收的转运常数（Kt）和最大转运速率（Vmax）。结果表明，盲肠的Kt和Vmax低于空肠。