Department of Natural Resources and Environmental Engineering, School of Agriculture, Shiraz University, Shiraz 71441- 65186, Iran.
Department of Natural Resources and Environmental Engineering, School of Agriculture, Shiraz University, Shiraz 71441- 65186, Iran.
Fish Shellfish Immunol. 2017 Oct;69:164-172. doi: 10.1016/j.fsi.2017.08.022. Epub 2017 Aug 19.
An 8- week feeding trial was conducted to investigate the effects of Ducrosia anethifolia essential oil on growth, blood immune parameters and immune related genes expression in juvenile rainbow trout (Oncorhynchus mykiss). Fish were allocated into 4 groups and fed on diet containing different levels of essential oil (0, 0.001, 0.01 and 0.1%) to apparent satiation in 30 min 3 times daily. Growth and immunological parameters were measured every ten days and tissue samples were taken from kidney and spleen on days 10, 30 and 50 to study the expression of IL-1β and TNF-α. The changes of measured parameters in different treatments and over sampling time series were statistically analysis based on repeated measurement method (P < 0.05). Results showed that growth did not affected by essential oil at different treatments. The highest level of hematocrit was observed in 0.001 treatment. The mean of RBC showed no significant differences among treatments. The mean of WBC in 0.01 and 0.1 groups were higher than those in 0.001 and control groups. Total protein, albumin and globulin and serum bactericidal activity showed no significant difference in different treatments. Neither treatments nor sampling times affected serum lysozyme activity. The highest mean of respiratory burst activity was observed in 0.01 group. The highest expression of both IL-1β and TNF-α genes in kidney was observed at 0.001 dose on day 30, while in spleen, the highest expression of IL-1β and TNF-α was obtained on day 30 at doses 0.1 and 0.01%, respectively. In conclusion the results of this study showed that feeding with lower and medium level of D. anethifolia for 30 days led to immunostimulatory effects in juvenile rainbow trout.
一项为期 8 周的饲养试验旨在研究土木香精油对幼虹鳟（Oncorhynchus mykiss）生长、血液免疫参数和免疫相关基因表达的影响。将鱼分为 4 组，分别投喂含不同水平精油（0、0.001、0.01 和 0.1%）的饲料，每天 3 次，30 分钟内饱食。每 10 天测量一次生长和免疫参数，并在第 10、30 和 50 天从肾脏和脾脏采集组织样本，研究 IL-1β 和 TNF-α 的表达。基于重复测量方法（P < 0.05），对不同处理和采样时间序列中测量参数的变化进行了统计分析。结果表明，在不同处理条件下，精油对生长没有影响。0.001 处理组的红细胞压积最高。各组间红细胞均值无显著差异。0.01 和 0.1 组的白细胞均值高于 0.001 和对照组。各组间总蛋白、白蛋白和球蛋白及血清杀菌活性均无显著差异。不同处理及采样时间均未影响血清溶菌酶活性。0.01 组呼吸爆发活性均值最高。在 0.001 剂量下，肾脏中 IL-1β 和 TNF-α 基因的表达在第 30 天最高，而在脾脏中，IL-1β 和 TNF-α 的表达在第 30 天最高，剂量分别为 0.1 和 0.01%。综上所述，本研究结果表明，幼虹鳟连续 30 天摄食低、中剂量土木香精油可产生免疫刺激作用。
