纳米黑色素-聚羟基丁酸酯（纳米黑色素-聚羟基丁酸酯）纳米复合材料薄膜的合成及其对生物膜形成的多药耐药金黄色葡萄球菌的保护作用。

Synthesis of Nm-PHB (nanomelanin-polyhydroxy butyrate) nanocomposite film and its protective effect against biofilm-forming multi drug resistant Staphylococcus aureus.

机构信息

Department of Food Science and Technology, Pondicherry University, Puducherry, 605014, India.

School of Microbiology, University College Cork, Cork, Ireland.

出版信息

Sci Rep. 2017 Aug 22;7(1):9167. doi: 10.1038/s41598-017-08816-y.

Melanin is a dark brown ubiquitous photosynthetic pigment which have many varied and ever expanding applications in fabrication of radio-protective materials, food packaging, cosmetics and in medicine. In this study, melanin production in a Pseudomonas sp. which was isolated from the marine sponge Tetyrina citirna was optimized employing one-factor at a time experiments and characterized for chemical nature and stability. Following sonication nucleated nanomelanin (Nm) particles were formed and evaluated for antibacterial and antioxidant properties. Nanocomposite film was fabricated using combinations (% w/v) of polyhydroxy butyrate-nanomelanin (PHB:Nm) blended with 1% glycerol. The Nm was found to be spherical in shape with a diameter of 100-140 nm and showed strong antimicrobial activity against both Gram positive and Gram negative bacteria. The Nm-PHB nanocomposite film was homogeneous, smooth, without any cracks, and flexible. XRD and DSC data indicated that the film was crystalline in nature, and was thermostable up to 281.87 °C. This study represents the first report on the synthesis of Nm and fabrication of Nm-PHB nanocomposite film which show strong protective effect against multidrug resistant Staphyloccoccus aureus. Thus this Nm-PHB nanocomposite film may find utility as packaging material for food products by protecting the food products from oxidation and bacterial contamination.

黑色素是一种深棕色的普遍存在的光合色素，在制造放射性保护材料、食品包装、化妆品和医学方面有许多不同的应用。在这项研究中，从海洋海绵 Tetyrina citirna 中分离出的假单胞菌中黑色素的生产得到了优化，采用单因素实验进行了优化，并对其化学性质和稳定性进行了表征。随后的超声处理引发了纳米黑色素（Nm）颗粒的形成，并对其抗菌和抗氧化性能进行了评估。使用聚羟基丁酸-纳米黑色素（PHB:Nm）与 1%甘油的组合（% w/v）制备了纳米复合材料薄膜。发现 Nm 呈球形，直径为 100-140nm，并对革兰氏阳性菌和革兰氏阴性菌均表现出强烈的抗菌活性。Nm-PHB 纳米复合材料薄膜均匀、光滑、无裂纹、柔韧性好。XRD 和 DSC 数据表明，该薄膜具有结晶性质，在 281.87°C 下热稳定性良好。本研究首次报道了 Nm 的合成和 Nm-PHB 纳米复合材料薄膜的制备，该薄膜对耐多药金黄色葡萄球菌表现出强烈的保护作用。因此，这种 Nm-PHB 纳米复合材料薄膜可用作食品包装材料，通过防止食品氧化和细菌污染来保护食品。