Department of Microbiology, Pondicherry University, Puducherry 605014, India.
J Nanobiotechnology. 2014 May 1;12:18. doi: 10.1186/1477-3155-12-18.
As a potent antimicrobial agent, silver nanostructures have been used in nanosensors and nanomaterial-based assays for the detection of food relevant analytes such as organic molecules, aroma, chemical contaminants, gases and food borne pathogens. In addition silver based nanocomposites act as an antimicrobial for food packaging materials. In this prospective, the food grade melanin pigment extracted from sponge associated actinobacterium Nocardiopsis alba MSA10 and melanin mediated synthesis of silver nanostructures were studied. Based on the present findings, antimicrobial nanostructures can be developed against food pathogens for food industrial applications.
Briefly, the sponge associated actinobacterium N. alba MSA10 was screened and fermentation conditions were optimized for the production of melanin pigment. The Plackett-Burman design followed by a Box-Behnken design was developed to optimize the concentration of most significant factors for improved melanin yield. The antioxidant potential, reductive capabilities and physiochemical properties of Nocardiopsis melanin was characterized. The optimum production of melanin was attained with pH 7.5, temperature 35°C, salinity 2.5%, sucrose 25 g/L and tyrosine 12.5 g/L under submerged fermentation conditions. A highest melanin production of 3.4 mg/ml was reached with the optimization using Box-Behnken design. The purified melanin showed rapid reduction and stabilization of silver nanostructures. The melanin mediated process produced uniform and stable silver nanostructures with broad spectrum antimicrobial activity against food pathogens.
The melanin pigment produced by N. alba MSA10 can be used for environmentally benign synthesis of silver nanostructures and can be useful for food packaging materials. The characteristics of broad spectrum of activity against food pathogens of silver nanostructures gives an insight for their potential applicability in incorporation of food packaging materials and antimicrobials for stored fruits and foods.
作为一种有效的抗菌剂，银纳米结构已被用于纳米传感器和基于纳米材料的分析物检测，例如有机分子、香气、化学污染物、气体和食源性病原体。此外，基于银的纳米复合材料可用作食品包装材料的抗菌剂。在这种情况下，研究了从海绵相关放线菌 Nocardiopsis alba MSA10 中提取的食品级黑色素色素以及黑色素介导的银纳米结构合成。根据目前的发现，可以针对食品病原体开发抗菌纳米结构，用于食品工业应用。
简而言之，筛选了海绵相关放线菌 N. alba MSA10，并优化了发酵条件以生产黑色素色素。采用 Plackett-Burman 设计和 Box-Behnken 设计来优化对黑色素产量影响最大的因素的浓度。表征了 Nocardiopsis 黑色素的抗氧化能力、还原能力和物理化学性质。在 35°C、盐度 2.5%、蔗糖 25g/L 和酪氨酸 12.5g/L 的条件下，在 pH 7.5 的最佳条件下，通过优化获得了最高的黑色素产量 3.4mg/ml。使用 Box-Behnken 设计进行优化后，黑色素产量达到了 3.4mg/ml。纯化的黑色素表现出快速还原和稳定银纳米结构的能力。黑色素介导的过程产生了均匀且稳定的银纳米结构，对食源性病原体具有广谱抗菌活性。
MSA10 产生的黑色素可用于环保型银纳米结构的合成，可用于食品包装材料。银纳米结构对食源性病原体具有广谱活性的特点，使其在食品包装材料和抗菌剂的整合方面具有潜在的应用前景，可用于储存水果和食品。
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