Department of Chemistry and Biochemistry, Florida International University, Miami, FL, 33199, USA.
Department of Chemistry and Biochemistry, Florida International University, Miami, FL, 33199, USA.
Anal Biochem. 2020 Apr 15;595:113619. doi: 10.1016/j.ab.2020.113619. Epub 2020 Feb 11.
In this note we describe a four-channel paper microfluidic device (μPAD) that utilizes gold nanoparticles (AuNPs) as colorimetric sensors and specifically tailored aptamers to capture a set of targeted drugs, including cocaine, codeine and methamphetamine. The design utilizes salt-induced aggregation of AuNPs to produce a color change that indicates the presence of target analytes. In the absence of the targets, the aptamers bind to AuNPs, preventing aggregation. This μPAD was developed by optimizing the concentrations of aggregating agents, AuNPs and aptamers. The resultant device is sensitive and specific, producing a positive result upon detection of the target analyte.
在本说明中，我们描述了一种四通道纸质微流控装置（μPAD），该装置利用金纳米粒子（AuNPs）作为比色传感器，并专门设计适配体来捕获一组靶向药物，包括可卡因、可待因和甲基苯丙胺。该设计利用盐诱导的 AuNPs 聚集产生颜色变化，从而指示目标分析物的存在。在没有目标的情况下，适配体与 AuNPs 结合，阻止聚集。该 μPAD 通过优化聚集剂、AuNPs 和适配体的浓度来开发。所得装置具有灵敏度和特异性，在检测到目标分析物时产生阳性结果。
