The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China.
Department of Oncology, The People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China.
Oncol Res. 2018 Mar 5;26(2):261-268. doi: 10.3727/096504017X15031557924132. Epub 2017 Aug 23.
Accumulating evidence has indicated that long noncoding RNA (lncRNA) PlncRNA-1 plays an important regulatory role in cancers. However, the expression and biological functions of PlncRNA-1 in colorectal cancer (CRC) are still unclear. In the present study, we determined the expression of PlncRNA-1 in CRC and explored the function of PlncRNA-1 on CRC cell progression. The results showed that PlncRNA-1 was significantly increased in CRC tissues and cell lines; high PlncRNA-1 expression was associated with depth of invasion, lymph node metastasis, and TNM stage of CRC patients. Kaplan-Meier curve analysis showed that patients with high PlncRNA-1 expression had a poor overall survival. PlncRNA-1 knockdown remarkably reduced cell proliferation, migration, and invasion and promoted cell apoptosis in vitro. In vivo xenograft experiments showed that PlncRNA-1 inhibition significantly suppressed tumor growth. Finally, we used an agonist (740Y-P) of the PI3K/Akt signaling pathway; function assays showed that PlncRNA-1 exerted its effects by targeting the PI3K/Akt signaling pathway in CRC. Taken together, our data suggested that PlncRNA-1 might act as an oncogene in CRC progression and serve as a potential biomarker and therapeutic target for the treatment of CRC.
越来越多的证据表明，长非编码 RNA（lncRNA）PlncRNA-1 在癌症中发挥着重要的调控作用。然而，PlncRNA-1 在结直肠癌（CRC）中的表达和生物学功能仍不清楚。在本研究中，我们测定了 PlncRNA-1 在 CRC 中的表达情况，并探讨了 PlncRNA-1 对 CRC 细胞进展的功能。结果表明，PlncRNA-1 在 CRC 组织和细胞系中显著上调；高 PlncRNA-1 表达与 CRC 患者的浸润深度、淋巴结转移和 TNM 分期相关。Kaplan-Meier 曲线分析显示，高 PlncRNA-1 表达的患者总体生存率较差。PlncRNA-1 敲低显著减少了细胞增殖、迁移和侵袭，并促进了细胞凋亡。体内异种移植实验表明，PlncRNA-1 抑制显著抑制了肿瘤生长。最后，我们使用了 PI3K/Akt 信号通路的激动剂（740Y-P）；功能分析表明，PlncRNA-1 通过靶向 CRC 中的 PI3K/Akt 信号通路发挥作用。综上所述，我们的数据表明，PlncRNA-1 可能在 CRC 进展中作为癌基因发挥作用，并可作为 CRC 治疗的潜在生物标志物和治疗靶点。
