Jia Gui-Qing, Zhang Ming-Ming, Wang Kang, Zhao Gao-Ping, Pang Ming-Hui, Chen Zhe-Yu
Department of Liver Surgery and Liver Transplantation Center, West China Hospital, Sichuan University, Chengdu, China.
Department of Gastrointestinal Surgery, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, China.
J Cell Biochem. 2018 Aug;119(8):7091-7104. doi: 10.1002/jcb.27031. Epub 2018 May 8.
Emerging evidence has identified that long non-coding RNAs (lncRNAs) may play an important role in the pathogenesis of many cancer types, including colorectal cancer (CRC). However, the role of PlncRNA-1 in CRC remains unclear. The aim of our present study was to investigate the potential functions of PlncRNA-1 in CRC and to identify the underlying mechanisms of action. We demonstrated that up-regulated PlncRNA-1 in CRC tissues and cells promoted cell proliferation by accelerating cell cycle process and inhibiting cell apoptosis in vitro, enhanced tumor growth and matastasis in vivo and was associated with cell migration and invasion, EMT process of CRC cells. In addition, PlncRNA-1 was a target of miR-204 and enhanced the expression of an endogenous miR-204 target, MMP9 in CRC cells. Furthermore, we found that PlncRNA-1 activates Wnt/β-catenin pathway through the miR-204 in CRC cells. These results suggest that the PlncRNA-1/miR-204/ Wnt/β-catenin regulatory network may shed light on tumorigenesis in CRC.
新出现的证据表明，长链非编码RNA（lncRNAs）可能在包括结直肠癌（CRC）在内的多种癌症类型的发病机制中发挥重要作用。然而，PlncRNA-1在结直肠癌中的作用仍不清楚。我们目前研究的目的是探讨PlncRNA-1在结直肠癌中的潜在功能，并确定其潜在的作用机制。我们证明，结直肠癌组织和细胞中上调的PlncRNA-1通过加速细胞周期进程和抑制体外细胞凋亡促进细胞增殖，增强体内肿瘤生长和转移，并与结直肠癌细胞的迁移、侵袭及上皮-间质转化（EMT）过程相关。此外，PlncRNA-1是miR-204的靶标，并增强了结直肠癌细胞中内源性miR-204靶标MMP9的表达。此外，我们发现PlncRNA-1通过结直肠癌细胞中的miR-204激活Wnt/β-连环蛋白信号通路。这些结果表明，PlncRNA-1/miR-204/Wnt/β-连环蛋白调控网络可能为结直肠癌的肿瘤发生提供线索。
