Department of Dermatology, Central Hospital of Xiangyang, Hubei College of Arts and Science, No. 136 Jingzhou Street, Xiangcheng District, Xiangyang, 441021, People's Republic of China.
Department of Dermatology, Jingzhou Central Hospital, The Second Clinical Medical College, Yangtze University, Jingzhou, 434100, People's Republic of China.
Mycopathologia. 2017 Dec;182(11-12):1037-1043. doi: 10.1007/s11046-017-0195-8. Epub 2017 Aug 23.
Scedosporium and Lomentospora species are the second most frequent colonizing, allergenic, or invasive fungal pathogens in patients with cystic fibrosis, and are responsible for infections varying from cutaneous and subcutaneous tissue infections caused by traumatic inoculation to severe systemic diseases in immunocompromised patients. The clinical relevance of fungal airway colonization for individual patients harboring Scedosporium and Lomentospora species is still an underestimated issue. The high resistance of Scedosporium and Lomentospora species to antifungal drugs has highlighted the need for alternative treatment modalities, and antimicrobial photodynamic therapy may be one such alternative. In this study, methylene blue was applied as a photosensitizing agent to 6 type strains of Scedosporium and Lomentospora species, and we irradiated the strains using a light-emitting diode (635 ± 10 nm, 12 J/cm). We evaluated the effects of photodynamic therapy on strain growth and on the in vitro susceptibility of the strains to itraconazole, voriconazole, posaconazole, and amphotericin B. A colony-forming unit reduction of up to 5.2 log was achieved. Minimal inhibitory concentration ranges also decreased significantly with photoinactivation. Photodynamic therapy improved both the inactivation rates and the antifungal susceptibility profile of all fungal isolates tested.
枝顶孢属和节菱孢属是囊性纤维化患者中第二常见的定植、致敏或侵袭性真菌病原体，可引起从创伤接种引起的皮肤和皮下组织感染到免疫功能低下患者的严重全身性疾病等各种感染。对于携带枝顶孢属和节菱孢属的个体患者，真菌气道定植的临床相关性仍然是一个被低估的问题。枝顶孢属和节菱孢属对抗真菌药物的高度耐药性突出表明需要替代治疗方式，而抗菌光动力疗法可能是一种替代方法。在这项研究中，亚甲蓝被用作光敏剂来处理 6 株枝顶孢属和节菱孢属的标准菌株，并用发光二极管（635 ± 10nm，12J/cm）对菌株进行照射。我们评估了光动力疗法对菌株生长以及体外对伊曲康唑、伏立康唑、泊沙康唑和两性霉素 B 的敏感性的影响。可实现高达 5.2log 的集落形成单位减少。最小抑菌浓度范围也随着光灭活而显著降低。光动力疗法改善了所有测试真菌分离物的失活率和抗真菌敏感性特征。
