Nemes Z, Adány R, Thomázy V
Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;52(5):453-66. doi: 10.1007/BF02889985.
Immunohistochemical techniques have been used to localize clotting factor XIII subunit A in human reactive lymphoid follicles. The follicular dendritic reticulum cells (DRCs) were identified by the monoclonal antibodies R4/23 and OKB-7 as well as by their 5'-nucleotidase positivity. Follicular histiocytic reticulum cells (HRCs) were demonstrated by their acid phosphatase and non-specific esterase reactions. Capillaries were selectively visualized by adenosine triphosphatase. The immunohistochemical demonstration of F-XIIIa was preferably carried out in combination with one or two of the above marker techniques, on the same cryostat section. The subunit A of factor XIII is present in follicular DRCs. Their selective immunohistochemical demonstration with antibody against F-XIIIa requires formaldehyde fixation of cryostat sections. Similar fixation, however, is inappropriate for the demonstration of F-XIIIa reactivity of DRCs in paraffin sections. For this purpose, acetic acid-formalin fixation is useful. Follicular HRCs are consistently negative for F-XIIIa, contrary to the F-XIIIa positivity of sinusoidal and interfollicular HRCs. Developmental and functional implications of F-XIIIa reactivity in DRCs and HRCs are suggested.
免疫组织化学技术已被用于在人类反应性淋巴滤泡中定位凝血因子 XIII 亚基 A。滤泡树突状网状细胞（DRCs）通过单克隆抗体 R4/23 和 OKB - 7 以及它们的 5'-核苷酸酶阳性来鉴定。滤泡组织细胞网状细胞（HRCs）通过其酸性磷酸酶和非特异性酯酶反应得以显示。毛细血管通过三磷酸腺苷酶被选择性地显示出来。F - XIIIa 的免疫组织化学显示最好在同一低温恒温器切片上与上述一种或两种标记技术联合进行。因子 XIII 的亚基 A 存在于滤泡 DRCs 中。用抗 F - XIIIa 抗体对其进行选择性免疫组织化学显示需要对低温恒温器切片进行甲醛固定。然而，类似的固定对于石蜡切片中 DRCs 的 F - XIIIa 反应性显示并不合适。为此，醋酸 - 甲醛固定是有用的。滤泡 HRCs 对 F - XIIIa 始终呈阴性，这与窦状和滤泡间 HRCs 的 F - XIIIa 阳性相反。文中提出了 DRCs 和 HRCs 中 F - XIIIa 反应性的发育和功能意义。
