Scientific Office, bioMérieux, La Balme Les Grottes, France.
Innovation Department, bioMérieux, La Balme Les Grottes, France.
Ann Lab Med. 2017 Nov;37(6):475-483. doi: 10.3343/alm.2017.37.6.475.
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized the identification of microbial species in clinical microbiology laboratories. MALDI-TOF-MS has swiftly become the new gold-standard method owing to its key advantages of simplicity and robustness. However, as with all new methods, adoption of the MALDI-TOF MS approach is still not widespread. Optimal sample preparation has not yet been achieved for several applications, and there are continuing discussions on the need for improved database quality and the inclusion of additional microbial species. New applications such as in the field of antimicrobial susceptibility testing have been proposed but not yet translated to the level of ease and reproducibility that one should expect in routine diagnostic systems. Finally, during routine identification testing, unexpected results are regularly obtained, and the best methods for transmitting these results into clinical care are still evolving. We here discuss the success of MALDI-TOF MS in clinical microbiology and highlight fields of application that are still amenable to improvement.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）彻底改变了临床微生物学实验室中微生物种属的鉴定方式。由于其简单、稳健的关键优势，MALDI-TOF-MS 已迅速成为新的金标准方法。然而，与所有新方法一样，MALDI-TOF MS 方法的采用仍不广泛。对于某些应用，尚未实现最佳的样本制备，并且还在持续讨论是否需要提高数据库质量并纳入更多微生物种属。已经提出了一些新的应用，如在抗菌药物敏感性测试领域，但尚未达到人们在常规诊断系统中所期望的那种简便性和可重复性。最后，在常规鉴定测试中，经常会得到意想不到的结果，而将这些结果传递到临床护理中的最佳方法仍在不断发展中。我们在这里讨论了 MALDI-TOF MS 在临床微生物学中的成功，并强调了仍有改进空间的应用领域。
