Department of Trace Element Analysis by Spectroscopy Method, Faculty of Chemistry, Adam Mickiewicz University in Poznan, Umultowska 89b, 61-614 Poznan, Poland.
Clinic of Geriatric Dentistry, Karol Marcinkowski University of Medical Sciences in Poznań, Bukowska 70, 60-812 Poznan, Poland.
Talanta. 2017 Dec 1;175:370-381. doi: 10.1016/j.talanta.2017.07.058. Epub 2017 Jul 21.
A new procedure for determination of elements derived from titanium implants and physiological elements in soft tissues by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented. The analytical procedure was developed which involved preparation of in-house matrix matched solid standards with analyte addition based on certified reference material (CRM) MODAS-4 Cormorant Tissue. Addition of gelatin, serving as a binding agent, essentially improved physical properties of standards. Performance of the analytical method was assayed and validated by calculating parameters like precision, detection limits, trueness and recovery of analyte addition using additional CRM - ERM-BB184 Bovine Muscle. Analyte addition was additionally confirmed by microwave digestion of solid standards and analysis by solution nebulization ICP-MS. The detection limits are in range 1.8μgg to 450μgg for Mn and Ca respectively. The precision values range from 7.3% to 42% for Al and Zn respectively. The estimated recoveries of analyte addition line within scope of 83%-153% for Mn and Cu respectively. Oral mucosa samples taken from patients treated with titanium dental implants were examined using developed analytical method. Standards and tissue samples were cryocut into 30µm thin sections. LA-ICP-MS allowed to obtain two-dimensional maps of distribution of elements in tested samples which revealed high content of Ti and Al derived from implants. Photographs from optical microscope displayed numerous particles with µm size in oral mucosa samples which suggests that they are residues from implantation procedure.
本文提出了一种通过激光烧蚀电感耦合等离子体质谱法（LA-ICP-MS）测定钛植入物衍生元素和软组织中生理元素的新方法。开发了一种分析程序，该程序涉及使用基于认证参考物质 (CRM) MODAS-4 Cormorant Tissue 的加标内标固体标准品进行制备。添加明胶作为结合剂，可显著改善标准品的物理性质。通过使用额外的 CRM-ERM-BB184 牛肌肉对分析物加标进行计算精密度、检测限、准确度和回收率等参数的评估和验证，来测试分析方法的性能。通过对固体标准品进行微波消解和溶液雾化 ICP-MS 分析，进一步确认了分析物的加标。对于 Mn 和 Ca，检测限分别在 1.8μg/g 到 450μg/g 范围内。Al 和 Zn 的精密度值分别在 7.3%到 42%范围内。对于 Mn 和 Cu，分析物加标线的估计回收率分别在 83%到 153%范围内。使用开发的分析方法检查了接受钛牙种植体治疗的患者的口腔黏膜样本。将标准品和组织样本冷冻切成 30μm 薄片。LA-ICP-MS 允许获得测试样本中元素分布的二维图谱，揭示了源自植入物的 Ti 和 Al 的高含量。光学显微镜的照片显示了口腔黏膜样本中具有 µm 大小的许多颗粒，这表明它们是植入过程的残留物。
