Abraham S N, Beachey E H
J Bacteriol. 1987 Jun;169(6):2460-5. doi: 10.1128/jb.169.6.2460-2465.1987.
Escherichia coli type 1 fimbriae are composed of subunits, each of which comprises 158 amino acids. We synthesized a copy of a 13-residue peptide, located near the NH2 terminus of the fimbrial subunit, that assumed some of the properties of type 1 fimbriae. At pH 5.5 the synthetic peptide autoassembled into fibrillar structures that resembled type 1 fimbriae except that they appeared less rigid and rodlike. A quaternary structure-specific monoclonal antibody against type 1 fimbriae recognized the synthetic peptide in the assembled but not the unassembled state. Furthermore, when the synthetic peptide was injected in its fimbrial conformation into rabbits, it evoked antibodies that reacted with type 1 fimbriae isolated from E. coli.
1型大肠杆菌菌毛由亚基组成，每个亚基包含158个氨基酸。我们合成了位于菌毛亚基NH2末端附近的一段13个残基的肽段，该肽段具有1型菌毛的一些特性。在pH 5.5时，合成肽自动组装成纤维状结构，类似于1型菌毛，只是它们看起来不那么坚硬和呈棒状。一种针对1型菌毛的四级结构特异性单克隆抗体在合成肽组装状态下能识别它，但在未组装状态下不能识别。此外，当将呈菌毛构象的合成肽注射到兔子体内时，它能诱发与从大肠杆菌分离出的1型菌毛发生反应的抗体。
