Stockholm University, Stress Research Institute, Stockholm, Sweden.
Karolinska Institutet, Department of Clinical Neuroscience, Stockholm, Sweden.
Sci Rep. 2017 Aug 25;7(1):9422. doi: 10.1038/s41598-017-09744-7.
Sleep deprivation has been reported to affect intrinsic brain connectivity, notably reducing connectivity in the default mode network. Studies to date have however shown inconsistent effects, in many cases lacked monitoring of wakefulness, and largely included young participants. We investigated effects of sleep deprivation on intrinsic brain connectivity in young and older participants. Participants aged 20-30 (final n = 30) and 65-75 (final n = 23) years underwent partial sleep deprivation (3 h sleep) in a cross-over design, with two 8-minutes eyes-open resting state functional magnetic resonance imaging (fMRI) runs in each session, monitored by eye-tracking. We assessed intrinsic brain connectivity using independent components analysis (ICA) as well as seed-region analyses of functional connectivity, and also analysed global signal variability, regional homogeneity, and the amplitude of low-frequency fluctuations. Sleep deprivation caused increased global signal variability. Changes in investigated resting state networks and in regional homogeneity were not statistically significant. Younger participants had higher connectivity in most examined networks, as well as higher regional homogeneity in areas including anterior and posterior cingulate cortex. In conclusion, we found that sleep deprivation caused increased global signal variability, and we speculate that this may be caused by wake-state instability.
睡眠剥夺已被报道会影响内在大脑连接，特别是减少默认模式网络的连接。然而，迄今为止的研究显示出不一致的效果，在许多情况下缺乏对清醒状态的监测，并且主要包括年轻参与者。我们研究了睡眠剥夺对年轻和老年参与者内在大脑连接的影响。参与者年龄在 20-30 岁（最终 n=30）和 65-75 岁（最终 n=23）之间，采用交叉设计进行部分睡眠剥夺（3 小时睡眠），在每个阶段进行两次 8 分钟的睁眼静息状态功能磁共振成像（fMRI）运行，通过眼动追踪进行监测。我们使用独立成分分析（ICA）以及功能连接的种子区域分析来评估内在大脑连接，还分析了全局信号变异性、区域同质性和低频波动的幅度。睡眠剥夺导致全局信号变异性增加。所研究的静息状态网络和区域同质性的变化没有统计学意义。年轻参与者在大多数检查的网络中具有更高的连接性，以及在前扣带和后扣带皮层等区域具有更高的区域同质性。总之，我们发现睡眠剥夺导致全局信号变异性增加，我们推测这可能是由于清醒状态不稳定所致。
