University of Rostock, Institute of Biological Sciences, Plant Physiology, A.-Einstein-Str. 3, D-18059 Rostock, Germany.
University of Freiburg, Faculty of Biology, Schänzlestr. 1, D-79104 Freiburg, Germany.
Curr Opin Biotechnol. 2018 Feb;49:94-99. doi: 10.1016/j.copbio.2017.07.008. Epub 2017 Aug 29.
Cyanobacteria are the only prokaryotes that perform oxygenic photosynthesis. Their evolutionary relation to plastids in eukaryotic phototrophs and their increasing utilization as green cell factories initiated the use of systems biology approaches early on. For select model strains, extensive 'omics' data sets have been generated, and genome-wide models have been elucidated. Moreover, the results obtained may be used for the optimization of cyanobacterial metabolism, which can direct the biotechnological production of biofuels or chemical feedstock. Synthetic biology approaches permit the rational construction of novel metabolic pathways that are based on the combination of multiple enzymatic activities of different origins. In addition, the manipulation of whole metabolic networks by CRISPR-based and sRNA-based technologies with multiple parallel targets will further stimulate the use of cyanobacteria for diverse applications in basic research and biotechnology.
蓝藻是唯一能进行产氧光合作用的原核生物。它们与真核光合生物中的质体在进化上的关系，以及它们作为绿色细胞工厂的日益普及，促使人们很早就开始采用系统生物学方法。对于一些选定的模式菌株，已经产生了广泛的“组学”数据集，并阐明了基因组范围的模型。此外，所获得的结果可用于优化蓝藻代谢，从而指导生物燃料或化学原料的生物技术生产。合成生物学方法允许基于不同来源的多种酶活性的组合来合理构建新型代谢途径。此外，通过基于 CRISPR 和基于 sRNA 的技术对多个平行靶标进行的整个代谢网络的操作，将进一步刺激蓝藻在基础研究和生物技术中的各种应用。
