Food Biosciences and Technology Program, Department of Agricultural and Environmental Sciences, Tennessee State University, Nashville, TN, 37209, USA.
Center for Excellence in Post-Harvest Technologies, North Carolina Research Campus, North Carolina A&T State University, Kannapolis, NC, USA.
Mycotoxin Res. 2017 Nov;33(4):343-350. doi: 10.1007/s12550-017-0291-0. Epub 2017 Aug 26.
In this proof-of-concept study, the efficacy of a medium-pressure UV (MPUV) lamp source to reduce the concentrations of aflatoxin B, aflatoxin B, and aflatoxin G (AFB AFB, and AFG) in pure water is investigated. Irradiation experiments were conducted using a collimated beam system operating between 200 to 360 nm. The optical absorbance of the solution and the irradiance of the lamp are considered in calculating the average fluence rate. Based on these factors, the UV dose was quantified as a product of average fluence rate and treatment time. Known concentrations of aflatoxins were spiked in water and irradiated at UV doses ranging from 0, 1.22, 2.44, 3.66, and 4.88 J cm. The concentration of aflatoxins was determined by HPLC with fluorescence detection. LC-MS/MS product ion scans were used to identify and semi-quantify degraded products of AFB, AFB, and AFG. It was observed that UV irradiation significantly reduced aflatoxins in pure water (p < 0.05). Irradiation doses of 4.88 J cm reduced concentrations 67.22% for AFG, 29.77% for AFB, and 98.25% for AFB (p < 0.05). Using this technique, an overall reduction of total aflatoxin content of ≈95% (p < 0.05) was achieved. We hypothesize that the formation of ˙OH radicals initiated by UV light may have caused photolysis of AFB, AFB, and AFG molecules. In cell culture studies, our results demonstrated that the increase of UV dosage decreased the aflatoxin-induced cytotoxicity in HepG cells. Therefore, our research finding suggests that UV irradiation can be used as an effective technique for the reduction of aflatoxins.
在这项概念验证研究中，研究了中压紫外线 (MPUV) 灯源降低纯水中黄曲霉毒素 B、B1 和 G（AFB1、AFB2 和 AFG）浓度的效果。使用在 200 至 360nm 之间运行的准直光束系统进行辐照实验。在计算平均辐照度时，考虑了溶液的光吸收率和灯的辐照度。基于这些因素，将紫外线剂量量化为平均辐照度和处理时间的乘积。已知浓度的黄曲霉毒素被加入水中，并在紫外线剂量为 0、1.22、2.44、3.66 和 4.88J/cm 时进行辐照。通过 HPLC 荧光检测确定黄曲霉毒素的浓度。使用 LC-MS/MS 产物离子扫描来鉴定和半定量 AFB1、AFB2 和 AFG 的降解产物。结果表明，紫外线辐照可显著降低纯水中的黄曲霉毒素（p<0.05）。4.88J/cm 的辐照剂量可将 AFG 的浓度降低 67.22%，AFB1 的浓度降低 29.77%，AFB2 的浓度降低 98.25%（p<0.05）。使用该技术，总黄曲霉毒素含量降低了约 95%（p<0.05）。我们假设紫外线光引发的·OH 自由基的形成可能导致 AFB1、AFB2 和 AFG 分子的光解。在细胞培养研究中，我们的结果表明，紫外线剂量的增加降低了黄曲霉毒素诱导的 HepG 细胞的细胞毒性。因此，我们的研究结果表明，紫外线辐照可作为降低黄曲霉毒素的有效技术。
