Goudarzi Afsaneh, Shiota Hitoshi, Rousseaux Sophie, Khochbin Saadi
Institut National de la Santé et de la Recherche Médicale U823 and Université Grenoble Alpes Institut Albert Bonniot, Grenoble F-38700, France.
Institut National de la Santé et de la Recherche Médicale U823 and Université Grenoble Alpes Institut Albert Bonniot, Grenoble F-38700, France.
J Mol Biol. 2014 Oct 9;426(20):3342-9. doi: 10.1016/j.jmb.2014.02.023. Epub 2014 Mar 5.
A genome-wide histone hyperacetylation is known to occur in the absence of transcription in haploid male germ cells, spermatids, before and during the global histone eviction and their replacement by non-histone DNA-packaging proteins. Although the occurrence of this histone hyperacetylation has been correlated with histone removal for a long time, the underlying mechanisms have remained largely obscure. Important recent discoveries have not only shed light on how histone acetylation could drive a subsequent transformation in genome organization but also revealed that the associated nucleosome dismantlement is a multi-step process, requiring the contribution of histone variants, critical destabilizing histone modifications and chromatin readers, including Brdt, working together to achieve the full packaging of the male genome, indispensable for the propagation of life.
已知在单倍体雄性生殖细胞（精子细胞）中，在全基因组组蛋白被清除并被非组蛋白DNA包装蛋白取代之前和期间，即使没有转录，全基因组范围的组蛋白超乙酰化也会发生。尽管这种组蛋白超乙酰化的发生长期以来一直与组蛋白的去除相关，但其潜在机制在很大程度上仍不清楚。最近的重要发现不仅揭示了组蛋白乙酰化如何推动基因组组织的后续转变，还表明相关的核小体拆解是一个多步骤过程，需要组蛋白变体、关键的使组蛋白不稳定的修饰以及染色质读取器（包括Brdt）的共同作用，以实现雄性基因组的完全包装，这对生命的繁衍至关重要。
