Department of Nutrition, Hebei Medical University, Shijiazhuang, 050017, China.
Department of Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang, 050017, China.
Sci Rep. 2017 Aug 29;7(1):9625. doi: 10.1038/s41598-017-10362-6.
The inability to successfully adapt to stress produces pathological changes that can lead to depression. Molecular hydrogen has anti-oxidative and anti-inflammatory activities and neuroprotective effects. However, the potential role of molecular hydrogen in stress-related disorders is still poorly understood. The present study aims to investigate the effects of hydrogen gas on resilience to stress in mice. The results showed that repeated inhalation of hydrogen-oxygen mixed gas [67%:33% (V/V)] significantly decreased both the acute and chronic stress-induced depressive- and anxiety-like behaviors of mice, assessed by tail suspension test (TST), forced swimming test (FST), novelty suppressed feeding (NSF) test, and open field test (OFT). ELISA analyses showed that inhalation of hydrogen-oxygen mixed gas blocked CMS-induced increase in the serum levels of corticosterone, adrenocorticotropic hormone, interleukin-6, and tumor necrosis factor-α in mice exposed to chronic mild stress. Finally, inhalation of hydrogen gas in adolescence significantly increased the resilience to acute stress in early adulthood, which illustrates the long-lasting effects of hydrogen on stress resilience in mice. This was likely mediated by inhibiting the hypothalamic-pituitary-adrenal axis and inflammatory responses to stress. These results warrant further exploration for developing molecular hydrogen as a novel strategy to prevent the occurrence of stress-related disorders.
无法成功适应压力会导致病理性变化，从而导致抑郁。分子氢具有抗氧化、抗炎和神经保护作用。然而，分子氢在应激相关疾病中的潜在作用仍知之甚少。本研究旨在探讨氢气对小鼠应激适应能力的影响。结果表明，重复吸入氢氧混合气体（67%:33%，V/V）可显著降低慢性轻度应激小鼠的急性和慢性应激诱导的抑郁样和焦虑样行为，通过悬尾试验（TST）、强迫游泳试验（FST）、新异物体抑制摄食试验（NSF）和旷场试验（OFT）进行评估。ELISA 分析表明，吸入氢氧混合气体可阻止 CMS 诱导的慢性轻度应激小鼠血清皮质酮、促肾上腺皮质激素、白细胞介素-6 和肿瘤坏死因子-α水平的升高。最后，青春期吸入氢气可显著提高成年早期急性应激的适应能力，这表明氢气对小鼠应激适应能力具有持久影响。这可能是通过抑制下丘脑-垂体-肾上腺轴和应激引起的炎症反应来实现的。这些结果值得进一步探索，以开发分子氢作为预防应激相关疾病发生的新策略。
