He Y, Shi J Z, Zhang R J, Zhai D X, Zhang D, Yu C Q, Liu Y H
1 Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University, Shanghai, China.
2 Graduate Management Unit, Changzheng Hospital, Second Military Medical University, Shanghai, China.
Reprod Sci. 2017 Feb;24(2):324-331. doi: 10.1177/1933719116655622. Epub 2016 Sep 27.
Oxidative stress is generated during the pathophysiology of endometriosis (EMT). Hydrogen (H) has been demonstrated as a gas antioxidant. The aim of the present study is to evaluate the protective effect of H on EMT in rats.
Sprague Dawley rats with surgically induced EMT were randomly received the inhalation of 67% H-33% oxygen (O) mixture (1 h/d, 4 weeks) immediately after the EMT surgery or 4 weeks after the operation. The mixture of 67% N-33% O was also used to exclude the possible influence of the increased O. Eight weeks after the operation, the endometrial tissues were weighted and analyzed using histology, immunohistochemistry, and real-time polymerase chain reaction. Several antioxidant enzymes and malondialdehyde were also measured in serum and tissue. The estrous cycles were monitored for H safety.
The results showed that both profiles of high-dose H breathing reduced the size of the endometrial explants, inhibited cell proliferation, improved superoxide dismutase, glutathione peroxidase, malondialdehyde, and catalase activities, and regulated the expression of matrix metalloproteinase 9 and cyclooxygenase 2. However, inhalation of the same dose of nitrogen failed to show the protection. High-dose H breathing did not change the normal estrous cyclicity.
These results suggest that 67% H-33% O breathing has a beneficial effect on EMT model rats, and inhalation of a high dose of H could be a potential method applied in clinical practice.
子宫内膜异位症（EMT）病理生理过程中会产生氧化应激。氢气（H）已被证明是一种气体抗氧化剂。本研究旨在评估H对大鼠EMT的保护作用。
手术诱导EMT的Sprague Dawley大鼠在EMT手术后立即或术后4周随机接受吸入67% H-33%氧气（O）混合物（1小时/天，共4周）。67%氮气-33%氧气的混合物也被用于排除增加的氧气可能产生的影响。术后8周，对子宫内膜组织进行称重，并使用组织学、免疫组织化学和实时聚合酶链反应进行分析。还测定了血清和组织中的几种抗氧化酶和丙二醛。监测动情周期以评估H的安全性。
结果显示，两种高剂量H吸入方案均减小了子宫内膜外植体的大小，抑制了细胞增殖，改善了超氧化物歧化酶、谷胱甘肽过氧化物酶、丙二醛和过氧化氢酶的活性，并调节了基质金属蛋白酶9和环氧化酶2的表达。然而，吸入相同剂量的氮气未显示出保护作用。高剂量H吸入未改变正常的动情周期。
这些结果表明，吸入67% H-33% O对EMT模型大鼠有有益作用，吸入高剂量H可能是一种可应用于临床实践的潜在方法。
