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使用快速接种系统评估厌氧微量稀释法的最低抑菌浓度(MIC)结果。

Evaluation of anaerobic microdilution MIC results with the Prompt Inoculation System.

作者信息

Mangels J I

出版信息

J Clin Microbiol. 1987 Jun;25(6):1140-2. doi: 10.1128/jcm.25.6.1140-1142.1987.

Abstract

The Prompt Inoculation System (3M Co.) was compared with the overnight suspension inoculation procedure used with a broth microdilution anaerobic commercial system (Micro-Media Systems) for differences in MIC. MIC results from both suspension methods using six National Committee for Clinical Laboratory Standards-recommended quality control organisms were identical in 18 instances (75%) and within +/- 1 log2 dilution in 96% of the comparisons. Results with 45 anaerobic clinical isolates also compared satisfactorily; 83% of the results were identical and 97% were within +/- 1 log2 dilution. In addition, the direct (Prompt)-inoculated microdilution trays produced better growth and more valid MIC results; 92% of the clinical isolates produced MIC results versus 79% by the overnight suspension procedure.

摘要

将即时接种系统(3M公司)与使用肉汤微量稀释厌氧商业系统(微媒体系统)的过夜悬液接种程序进行比较,以观察最低抑菌浓度(MIC)的差异。使用六种美国国家临床实验室标准委员会推荐的质量控制微生物,两种悬液法的MIC结果在18例(75%)中相同,在96%的比较中相差不超过±1个log2稀释度。对45株厌氧临床分离株的检测结果也比较令人满意;83%的结果相同,97%相差不超过±1个log2稀释度。此外,直接(即时)接种的微量稀释板生长更好,MIC结果更有效;92%的临床分离株得出了MIC结果,而过夜悬液法为79%。

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本文引用的文献

4
Preparing inoculum for susceptibility testing of anaerobes.制备用于厌氧菌药敏试验的接种物。
J Clin Microbiol. 1984 Mar;19(3):321-5. doi: 10.1128/jcm.19.3.321-325.1984.
5
Inoculum preparation for anaerobic susceptibility tests.厌氧药敏试验的接种物制备。
J Clin Microbiol. 1983 Sep;18(3):733-4. doi: 10.1128/jcm.18.3.733-734.1983.

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