Swenson J M, Thornsberry C
J Clin Microbiol. 1984 Mar;19(3):321-5. doi: 10.1128/jcm.19.3.321-325.1984.
We studied six methods for preparation of inoculum to use in susceptibility tests of anaerobic bacteria to determine viable counts of 15 different species of anaerobes. We counted viable bacteria for each method-organism combination. Methods studied included those used for the more routine tests (broth microdilution and broth-disk elution) and for the National Committee for Clinical Laboratory Standards standard reference agar method. Inocula prepared from broth suspensions of organisms from 24- and 48-h anaerobe blood agar plates and adjusted to the turbidity of a 0.5 McFarland standard gave the most consistent counts for all organisms and also the highest numbers. Counts from these suspensions were higher than those from overnight growth in thioglycolate or Schaedler broth when all were adjusted against the same turbidity standard. Preparing inoculum directly from agar plates may also speed up the sometimes lengthy process of susceptibility testing of anaerobes and thus make results more clinically useful.
我们研究了六种制备接种物的方法，用于厌氧菌药敏试验，以确定15种不同厌氧菌的活菌数。我们对每种方法与微生物的组合进行了活菌计数。所研究的方法包括用于更常规试验的方法（肉汤微量稀释法和肉汤纸片洗脱法）以及美国国家临床实验室标准委员会的标准参考琼脂法。用来自24小时和48小时厌氧菌血琼脂平板上的微生物肉汤悬液制备接种物，并将其调整至0.5麦氏标准浊度，对所有微生物而言，所得计数最为一致，数量也最高。当所有悬液都根据相同的浊度标准进行调整时，这些悬液的计数高于硫乙醇酸盐或 Schaedler 肉汤过夜培养物的计数。直接从琼脂平板制备接种物也可能加快厌氧菌药敏试验有时冗长的过程，从而使结果在临床上更有用。
