检测人粪便样本中的产气荚膜梭菌肠毒素及血清中的抗肠毒素。
Detection of Clostridium perfringens enterotoxin in human fecal samples and anti-enterotoxin in sera.
作者信息
Naik H S, Duncan C L
出版信息
J Clin Microbiol. 1978 Apr;7(4):337-40. doi: 10.1128/jcm.7.4.337-340.1978.
Abstract
By using counterimmunoelectrophoresis (CIEP), Clostridium perfringens enterotoxin was successfully demonstrated in fecal samples collected within 1 day of attack from sick individuals involved in a bacteriologically and epidemiologically proven outbreak of C. perfringens food poisoning. In contrast, enterotoxin was not demonstrable in fecal samples of apparently healthy individuals both at high- and low-risk exposure to the organism and enterotoxin or in fecal samples collected 4 to 5 days after a food poisoning outbreak. A 100% prevalence of C. perfringens anti-enterotoxin in sera of human volunteers at high- as well as low-risk exposure to the organism and enterotoxin was recorded with CIEP.
摘要
通过使用对流免疫电泳（CIEP），在参与产气荚膜梭菌食物中毒细菌学和流行病学确诊暴发的患病个体发病后1天内采集的粪便样本中成功检测到产气荚膜梭菌肠毒素。相比之下，在高风险和低风险接触该菌及肠毒素的明显健康个体的粪便样本中，以及在食物中毒暴发后4至5天采集的粪便样本中，均未检测到肠毒素。通过CIEP检测发现，高风险和低风险接触该菌及肠毒素的人类志愿者血清中产气荚膜梭菌抗肠毒素的阳性率均为100%。
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