Wu Zhengke, Liu Jiao, Chen Jiang, Pirzado Shoaib Ahmed, Li Yang, Cai Huiyi, Liu Guohua
Key Laboratory of Feed Biotechnology of Agricultural Ministry and Rural Affairs, National Engineering Research Center of Biological Feed, Feed Research Institute of Chinese Academy of Agricultural Science, Beijing 100081, China.
Department of Animal Nutrition, Sindh Agriculture University, Tnadojam 70060, Pakistan.
Animals (Basel). 2020 Oct 1;10(10):1774. doi: 10.3390/ani10101774.
Rapeseed meal (RSM) is a common protein ingredient in animal diets, while the proportion of RSM in diets is limited because of its anti-nutritional factors. Fermentation based on mixed microbial strains appears to be a suitable approach to improve the nutritive value of rapeseed meal in animal feed. In this study, we evaluated the effects of fermentation on the apparent metabolizable energy (AME) values and standardized ileal digestibility (SID) of amino acids in RSM fed broilers. The AME and nitrogen-corrected apparent metabolizable energy (AMEn) values of RSM and fermented rapeseed meal (FRSM) were determined by the substitution method, with RSM and FRSM proportionally replacing the energy-yielding components of the basal diet by 30%. Results show that fermentation improved AME and AMEn of RSM from 7.44 to 8.51 MJ/kg and from 7.17 to 8.26 MJ/kg, respectively. In the second experiment, two experimental diets were formulated, with RSM and FRSM being the sole sources of amino acids. A nitrogen-free diet (NFD) was also formulated to determine endogenous amino acids losses (EAAL). Feeding on FRSM resulted in higher ( < 0.05) apparent ileal digestibility (AID) and SID of alanine, valine, isoleucine, leucine, tyrosine, lysine, arginine, and phenylalanine. No significant differences between RSM and FRSM were found for AID and SID of asparagine, histidine, threonine, serine, glutamine, praline, glycine, methionine, and cystine. FRSM had greater AMEn values and SID of amino acids compared to RSM, therefore, FRSM was nutritionally superior to RSM in broiler diets.
菜籽粕(RSM)是动物日粮中常见的蛋白质成分,然而由于其抗营养因子,日粮中菜籽粕的比例受到限制。基于混合微生物菌株的发酵似乎是提高动物饲料中菜籽粕营养价值的合适方法。在本研究中,我们评估了发酵对饲喂菜籽粕的肉鸡中氨基酸的表观代谢能(AME)值和标准回肠消化率(SID)的影响。通过替代法测定了菜籽粕和发酵菜籽粕(FRSM)的AME和氮校正表观代谢能(AMEn)值,将菜籽粕和发酵菜籽粕按比例替代基础日粮中30%的能量产生成分。结果表明,发酵使菜籽粕的AME和AMEn分别从7.44 MJ/kg提高到8.51 MJ/kg,从7.17 MJ/kg提高到8.26 MJ/kg。在第二个实验中,配制了两种实验日粮,以菜籽粕和发酵菜籽粕作为氨基酸的唯一来源。还配制了无氮日粮(NFD)以确定内源性氨基酸损失(EAAL)。饲喂发酵菜籽粕导致丙氨酸、缬氨酸、异亮氨酸、亮氨酸、酪氨酸、赖氨酸、精氨酸和苯丙氨酸的表观回肠消化率(AID)和SID更高(<0.05)。对于天冬酰胺、组氨酸、苏氨酸、丝氨酸、谷氨酰胺、脯氨酸、甘氨酸、蛋氨酸和胱氨酸的AID和SID,菜籽粕和发酵菜籽粕之间未发现显著差异。与菜籽粕相比,发酵菜籽粕具有更高的AMEn值和氨基酸SID,因此,在肉鸡日粮中,发酵菜籽粕在营养上优于菜籽粕。