Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA; Department of Virology, University of Helsinki, Helsinki, Finland.
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX, USA.
Virus Res. 2017 Oct 15;242:49-57. doi: 10.1016/j.virusres.2017.08.012. Epub 2017 Sep 21.
Eleven viral isolates derived mostly in albopictus C6/36 cells from mosquito pools collected in Southeast Asia and the Americas between 1966 and 2014 contained particles with electron microscopy morphology typical of reoviruses. Metagenomics analysis yielded the near complete genomes of three novel reoviruses, Big Cypress orbivirus, Ninarumi virus, and High Island virus and a new tetravirus, Sarawak virus. Strains of previously characterized Sathuvarachi, Yunnan, Banna and Parry's Lagoon viruses (Reoviridae), Bontang virus (Mesoniviridae), and Culex theileri flavivirus (Flaviviridae) were also characterized. The availability of these mosquito virus genomes will facilitate their detection by metagenomics or PCR to better determine their geographic range, extent of host tropism, and possible association with arthropod or vertebrate disease.
从 1966 年至 2014 年期间在东南亚和美洲的蚊虫池中采集的大多数源自白纹伊蚊 C6/36 细胞的 11 个病毒分离株含有电子显微镜形态典型的呼肠孤病毒的颗粒。宏基因组分析产生了三种新型呼肠孤病毒、大柏树 orbivirus、尼那鲁米病毒和海伊岛病毒以及一种新的四病毒、沙捞越病毒的近乎完整基因组。先前表征的 Sathuvarachi、云南、邦坦和帕里泻湖病毒（呼肠孤病毒科）、邦坦病毒（ mesoniviridae 科）和库蚊 Theileri 黄病毒（黄病毒科）的毒株也得到了表征。这些蚊虫病毒基因组的可用性将通过宏基因组学或 PCR 检测来促进，以更好地确定它们的地理范围、宿主嗜性的程度以及与节肢动物或脊椎动物疾病的可能关联。
