Rao P Sailaja, Mohan G Krishna
Department of Pharmacology, Sri Venkateshwara College of Pharmacy & Research Centre, Madhapur, Hyderabad 500081, Telangana, India.
Centre for Pharmaceutical Sciences, JNTUH, Hyderabad, Telangana, India.
Saudi J Biol Sci. 2017 Sep;24(6):1262-1267. doi: 10.1016/j.sjbs.2016.01.010. Epub 2016 Jan 11.
Roxb. Commonly known as "Kakora" in Telugu, is used in the Indian traditional system of medicine for the treatment of diabetes. The aim of this study was to investigate the antidiabetic activity of methanolic extract of seeds (MEMD) in streptozotocin (STZ) induced diabetic rats. The α-amylase inhibitory activity of the MEMD was done by spectrophotometric method. Diabetes was induced by STZ (45 mg/kg; i.p), MEMD (100 & 200 mg/kg; b.wt) and standard drug metformin (50 mg/kg; b.wt) were administered to the diabetic rats. Blood glucose was estimated on the 11th day and the level of MDA, SOD and CAT was estimated in the liver tissue homogenate after the 15 days of experimental period. MEMD showed significant inhibition of alpha amylase activity and the IC was found to be 48 μg/ml. Oral administration of MEMD significantly reduced blood glucose level ( < 0.05), diminished the MDA level and refurbished depleted antioxidant enzymes and Insulin level to normalcy. These findings revealed that seeds possess antihyperglycemic, antioxidant and anti lipid peroxidative activity and thus mitigate STZ-induced oxidative damage.
罗克斯伯。在泰卢固语中通常被称为“卡科拉”，在印度传统医学中用于治疗糖尿病。本研究的目的是调查种子甲醇提取物（MEMD）对链脲佐菌素（STZ）诱导的糖尿病大鼠的抗糖尿病活性。通过分光光度法测定MEMD的α-淀粉酶抑制活性。用STZ（45mg/kg；腹腔注射）诱导糖尿病，给糖尿病大鼠给予MEMD（100和200mg/kg；体重）和标准药物二甲双胍（50mg/kg；体重）。在第11天估计血糖，并在实验期15天后估计肝组织匀浆中MDA、SOD和CAT的水平。MEMD显示出对α-淀粉酶活性的显著抑制，IC50为48μg/ml。口服MEMD显著降低血糖水平（P<0.05），降低MDA水平，并使耗尽的抗氧化酶和胰岛素水平恢复正常。这些发现表明，种子具有降血糖、抗氧化和抗脂质过氧化活性，从而减轻STZ诱导的氧化损伤。
