Fagliarone Claudia, Mosca Claudia, Ubaldi Ilaria, Verseux Cyprien, Baqué Mickael, Wilmotte Annick, Billi Daniela
Department of Biology, University of Rome Tor Vergata, Rome, Italy.
Astrobiological Laboratories, Institute of Planetary Research, Management and Infrastructure, German Aerospace Center, Berlin, Germany.
Extremophiles. 2017 Nov;21(6):981-991. doi: 10.1007/s00792-017-0957-8. Epub 2017 Aug 30.
To investigate the relationship between desiccation and the extent of protein oxidation in desert strains of Chroococcidiopsis a selection of 10 isolates from hot and cold deserts and the terrestrial cyanobacterium Chroococcidiopsis thermalis sp. PCC 7203 were exposed to desiccation (air-drying) and analyzed for survival. Strain CCMEE 029 from the Negev desert and the aquatic cyanobacterium Synechocystis sp. PCC 6803 were further investigated for protein oxidation after desiccation (drying over silica gel), treatment with HO up to 1 M and exposure to γ-rays up to 25 kGy. Then a selection of desert strains of Chroococcidiopsis with different survival rates after prolonged desiccation, as well as Synechocystis sp. PCC 6803 and Chroococcidiopsis thermalis sp. PCC 7203, were analyzed for protein oxidation after treatment with 10 and 100 mM of HO. Results suggest that in the investigated strains a tight correlation occurs between desiccation and radiation tolerance and avoidance of protein oxidation.
为了研究干燥与嗜热栖热蓝细菌荒漠菌株中蛋白质氧化程度之间的关系，从炎热和寒冷沙漠中选取了10株嗜热栖热蓝细菌分离株以及陆地蓝细菌嗜热栖热蓝细菌菌株PCC 7203，使其暴露于干燥环境（风干）中并分析其存活率。对来自内盖夫沙漠的CCMEE 029菌株和水生蓝细菌聚球藻属菌株PCC 6803在干燥处理（用硅胶干燥）、用高达1 M的过氧化氢处理以及暴露于高达25 kGy的γ射线后，进一步研究其蛋白质氧化情况。然后，对经过长时间干燥后具有不同存活率的一系列嗜热栖热蓝细菌荒漠菌株，以及聚球藻属菌株PCC 6803和嗜热栖热蓝细菌菌株PCC 7203，在用10 mM和100 mM过氧化氢处理后分析其蛋白质氧化情况。结果表明，在所研究的菌株中，干燥与辐射耐受性以及避免蛋白质氧化之间存在紧密的相关性。
