Guduff Ludmilla, Kurzbach Dennis, van Heijenoort Carine, Abergel Daniel, Dumez Jean-Nicolas
Institut de Chimie des Substances Naturelles, CNRS UPR2301, Univ. Paris Sud, Université Paris-Saclay, 91190, Gif-sur-Yvette, France.
Laboratoire des Biomolécules, Département de chimie, Ecole normale supérieure, UPMC Univ. Paris 06, CNRS, PSL Research University, 75005, Paris, France.
Chemistry. 2017 Nov 27;23(66):16722-16727. doi: 10.1002/chem.201703300. Epub 2017 Sep 22.
Diffusion-ordered NMR spectroscopy (DOSY) is a powerful approach for the analysis of molecular mixtures, yet its application range is limited by the relatively low sensitivity of NMR. We show here that spectrally resolved C DOSY data can be collected, in a single scan, for substrates hyperpolarised by dissolution dynamic nuclear polarisation (D-DNP), which provides signal enhancements of several orders of magnitude. For this we use a convection-compensation pulse scheme, which we also analyse by numerical simulation. The proposed method further allows the acquisition of several consecutive DOSY spectra in a single D-DNP experiment.
扩散排序核磁共振波谱法（DOSY）是分析分子混合物的一种强大方法，但其应用范围受到核磁共振相对较低灵敏度的限制。我们在此表明，对于通过溶解动态核极化（D-DNP）超极化的底物，可以在单次扫描中收集光谱分辨的碳DOSY数据，D-DNP可提供几个数量级的信号增强。为此，我们使用了一种对流补偿脉冲序列，我们还通过数值模拟对其进行了分析。所提出的方法还允许在单个D-DNP实验中采集多个连续的DOSY光谱。
