Department of General Biology, Biological Sciences Center, State University of Londrina - UEL, Londrina, Brazil.
Department of Pathological Sciences, Biological Sciences Center, State University of Londrina - UEL, Londrina, Brazil.
Basic Clin Pharmacol Toxicol. 2018 Feb;122(2):262-270. doi: 10.1111/bcpt.12894. Epub 2017 Sep 19.
Bisphenol A (BPA) is a synthetic non-steroidal oestrogen used in the production of plastics. BPA can cause alterations in the endocrine system of human beings and animals at varied stages of development. During puberty, altered morphological, sexual behaviour and completion of the epididymal development occur. Therefore, this study aimed to evaluate the effects of BPA on epididymal development during the peripubertal period of rats. Male Wistar rats were treated with BPA via gavage at doses of 20 μg/kg or 200 μg/kg per day [post-natal day (PND] 36-66). The control group received the vehicles under the same conditions. Feed and water were provided ad libitum. On PND 67, the epididymis was removed, weighed, divided into caput/corpus and cauda sections. It was then used for sperm count determination; histopathological and stereological evaluation; inflammatory cell enzymatic profiling (myeloperoxidase activity - MPO; N-acetylglucosaminidase - NAG); immunohistochemistry for IL-6; and evaluation of superoxide anion levels and malondialdehyde (MDA). Exposure to BPA at 200 μg/kg caused a significant increase of MPO activity and immunoreactivity to IL-6 (interleukin-6) as well as remodelling of tissue components in the caput/corpus and cauda regions of the epididymis. Under these experimental conditions, it is concluded that BPA alters post-natal epididymal development.
双酚 A（BPA）是一种用于生产塑料的合成非甾体雌激素。BPA 可以在人类和动物的不同发育阶段改变内分泌系统。在青春期，会发生形态学、性行为改变和附睾发育完成。因此，本研究旨在评估 BPA 对青春期大鼠附睾发育的影响。雄性 Wistar 大鼠通过灌胃接受 20μg/kg 或 200μg/kg 剂量的 BPA[产后第 36-66 天（PND）]。对照组在相同条件下接受载体。自由提供饲料和水。在 PND67 时，取出附睾，称重，分为头部/体部和尾部。然后用于精子计数测定；组织病理学和体视学评估；炎症细胞酶谱分析（髓过氧化物酶活性-MPO；N-乙酰氨基葡萄糖苷酶-NAG）；IL-6 的免疫组织化学；以及超氧阴离子水平和丙二醛（MDA）的评估。暴露于 200μg/kg 的 BPA 会导致 MPO 活性和 IL-6（白细胞介素 6）免疫反应显著增加，以及附睾头部/体部和尾部组织成分的重塑。在这些实验条件下，可以得出结论，BPA 改变了产后附睾发育。
