Department of Physiology and Pharmacology, Faculty of Medicine of the Federal University of Ceará (UFC), Rua Cel. Nunes de Melo, 1000, Fortaleza, CEP 60416-000, Ceará, Brazil.
G & F Spirulina do Brasil Ltda., Fortaleza, Brazil.
Neurochem Res. 2017 Dec;42(12):3390-3400. doi: 10.1007/s11064-017-2379-5. Epub 2017 Aug 31.
Spirulina platensis (SPI) is a cyanobacterium, presenting anti-inflammatory and antioxidant actions. Considering the importance of inflammation and oxidative stress in Parkinson's disease (PD), SPI neuroprotective effects were evaluated in a model of PD. Male Wistar rats were divided into: sham-operated (SO), untreated 6-OHDA and 6-OHDA treated with SPI (25 and 50 mg/kg, p.o.). The 6-OHDA was injected into the right striata and SPI treatments started 24 h later for 2 weeks. The SO and untreated 6-OHDA-lesioned groups were administered with distilled water, for the same period. Afterwards, the animals were subjected to the apomorphine-induced rotational test and euthanized for striatal measurements of DA and DOPAC, nitrite and TBARS and immunohistochemistry assays for TH, DAT, iNOS and COX-2. SPI reduced the apomorphine-induced rotational behavior, DA and DOPAC depletions and nitrite and TBARS increases, at its high dose. Furthermore, TH and DAT immunoreactivities in the lesioned striatum of the untreated 6-OHDA-lesioned group were attenuated by SPI. Similarly, immunoreactivities for iNOS and COX-2 were also decreased after SPI treatments. In conclusion, we showed that behavioral and neurochemical alterations in hemiparkinsonian rats were partly reversed by SPI, characterizing the neuroprotective potential of Spirulina and stimulating translational studies focusing on its use as an alternative treatment for PD.
钝顶螺旋藻（SPI）是一种蓝藻，具有抗炎和抗氧化作用。鉴于炎症和氧化应激在帕金森病（PD）中的重要性，评估了 SPI 对 PD 模型的神经保护作用。雄性 Wistar 大鼠分为：假手术（SO）、未治疗的 6-OHDA 和 6-OHDA 用 SPI（25 和 50mg/kg，po）治疗。6-OHDA 被注射到右侧纹状体，SPI 治疗在 24 小时后开始，持续 2 周。SO 和未治疗的 6-OHDA 损伤组给予蒸馏水，持续相同时间。之后，动物进行阿扑吗啡诱导的旋转试验，然后处死进行纹状体 DA 和 DOPAC、亚硝酸盐和 TBARS 的测量以及 TH、DAT、iNOS 和 COX-2 的免疫组织化学检测。SPI 降低了高剂量时阿扑吗啡诱导的旋转行为、DA 和 DOPAC 的耗竭以及亚硝酸盐和 TBARS 的增加。此外，SPI 还减弱了未治疗的 6-OHDA 损伤组损伤纹状体中 TH 和 DAT 的免疫反应性。同样，SPI 治疗后 iNOS 和 COX-2 的免疫反应性也降低。总之，我们表明，半帕金森大鼠的行为和神经化学改变部分被 SPI 逆转，这表明螺旋藻具有神经保护潜力，并刺激了专注于将其用作 PD 替代治疗的转化研究。
