Graduate Program in Morphofunctional Sciences, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Graduate Program in Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Naunyn Schmiedebergs Arch Pharmacol. 2020 Jun;393(6):1077-1088. doi: 10.1007/s00210-019-01793-1. Epub 2020 Jan 15.
Linalool (LIN) is a monoterpene, responsible for the aroma of essential oils in some species. It presents a sedative and anxiolytic potential, enhancing GABAergic currents and behaving as a benzodiazepine-type of drug. The objectives of the present work were to study the neuroprotective effects of LIN on a model of Parkinson's disease. For that, male Wistar rats were divided into the following groups: sham-operated (SO), 6-OHDA-lesioned, and 6-OHDA-lesioned and treated with LIN (25, 50, and 100 mg/kg, p.o.) for 2 weeks. Afterwards, the animals were subjected to behavioral tests (apomorphine-induced rotations, open field, and forced swimming tests). Then, the animals were euthanized, and the striatum, hippocampus, and prefrontal cortex were processed for neurochemistry (nitrite and lipoperoxidation measurements) and immunohistochemistry (TH and DAT) assays. The results were analyzed by ANOVA and Tukey's test for multiple comparisons and considered significant at p < 0.05. LIN significantly improved the behavioral alterations of the 6-OHDA-lesioned group, as evaluated by the apomorphine-induced rotations, open field, and forced swimming tests. In addition, LIN partially reversed the decreased DA, DOPAC, and HVA contents observed in the 6-OHDA-lesioned striatum. The untreated 6-OHDA group presented increased nitrite contents and lipoperoxidation in all the brain areas studied, and these changes were completely reversed after LIN treatments. Finally, LIN significantly prevented the reduction in TH and DAT expressions demonstrated in the right 6-OHDA-lesioned striatum. All these data strongly suggest that LIN presents a neuroprotective action in hemiparkinsonian rats, probably related to the drug anti-inflammatory and antioxidant activities.
芳樟醇（LIN）是一种单萜，负责某些物种精油的香气。它具有镇静和抗焦虑作用，增强 GABA 能电流，并表现出苯二氮䓬类药物的作用。本工作的目的是研究 LIN 对帕金森病模型的神经保护作用。为此，雄性 Wistar 大鼠分为以下几组：假手术（SO）、6-OHDA 损伤和 6-OHDA 损伤并用 LIN（25、50 和 100mg/kg，po）治疗 2 周。然后，对动物进行行为测试（阿扑吗啡诱导旋转、旷场和强迫游泳测试）。然后，处死动物，处理纹状体、海马体和前额叶皮层进行神经化学（亚硝酸盐和脂质过氧化测量）和免疫组织化学（TH 和 DAT）测定。结果通过 ANOVA 和 Tukey 检验进行多重比较分析，认为 p<0.05 具有统计学意义。LIN 显著改善了 6-OHDA 损伤组的行为改变，如阿扑吗啡诱导旋转、旷场和强迫游泳测试评估所示。此外，LIN 部分逆转了 6-OHDA 损伤纹状体中 DA、DOPAC 和 HVA 含量的降低。未经治疗的 6-OHDA 组在所有研究的脑区均表现出亚硝酸盐含量增加和脂质过氧化，LIN 治疗后这些变化完全逆转。最后，LIN 显著防止了右 6-OHDA 损伤纹状体中 TH 和 DAT 表达的减少。所有这些数据强烈表明，LIN 对偏侧帕金森病大鼠具有神经保护作用，可能与其药物抗炎和抗氧化活性有关。
