Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University, S366, Jiangnan Boulevard, Guangzhou, 510280, China.
Department of Periodontology, Stomatological Hospital, Southern Medical University, Guangzhou, China.
Clin Oral Investig. 2018 Mar;22(2):1103-1111. doi: 10.1007/s00784-017-2194-z. Epub 2017 Aug 31.
The main purpose of this study was to investigate the detailed healing process of the roots and surrounding periodontium [cementum, periodontal ligament (PDL), and bone] at different time points after intentional root damage with miniscrew implants (MSIs).
After cone-beam computed tomography examination and measurement, a total of 78 premolar and molar roots from five beagle dogs were intentionally damaged by implanting miniscrews in the interradicular region. MSIs were immediately removed, and the histological morphology was observed at days 0 and 3 and at weeks 1, 2, 3, 4, 6, 8, and 12 after root injury using haematoxylin and eosin and fluorescence stainings (fluorescence staining was performed at days 28 and 56).
An early new attachment of PDL adhering on to the damaged root surface was found at week 2 after root injury. Tissue differentiation of newly formed bone tissue, PDL, and cementum began at week 3. Moreover, the newly formed cementum and bone were constantly forming and mineralising at weeks 4, 6, 8, and 12, and the width of PDL gradually narrowed until close to the normal width at week 12.
This study demonstrated the complete healing process of the roots and surrounding periodontium after root damage with MSIs in dogs when the damage was limited to the cementum or dentin.
The findings of this study may help provide a better understanding of the detailed healing process in roots and PDLs damaged by MSIs.
本研究的主要目的是研究在使用微螺钉植入物（MSIs）故意损伤牙根后不同时间点牙根和周围牙周组织（牙骨质、牙周韧带 [PDL] 和骨）的详细愈合过程。
在锥形束计算机断层扫描检查和测量后，将来自五只比格犬的总共 78 颗前磨牙和磨牙牙根通过在根间区域植入微螺钉来故意损伤。立即取出 MSIs，并在损伤后第 0 天和第 3 天以及第 1、2、3、4、6、8 和 12 周使用苏木精和伊红及荧光染色（第 28 天和第 56 天进行荧光染色）观察组织形态学。
在损伤后第 2 周发现新的 PDL 附着在受损牙根表面的早期新附着。在第 3 周开始出现新形成的骨组织、PDL 和牙骨质的组织分化。此外，新形成的牙骨质和骨在第 4、6、8 和 12 周不断形成和矿化，PDL 的宽度逐渐变窄，直到第 12 周接近正常宽度。
本研究在狗中证明了在 MSIs 损伤仅限于牙骨质或牙本质时牙根和周围牙周组织的完全愈合过程。
本研究的结果可能有助于更好地了解 MSIs 损伤的牙根和 PDL 的详细愈合过程。
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