从人阴道中分离的唾液乳杆菌 MG242 的体外益生菌特性。
In Vitro Probiotic Properties of Lactobacillus salivarius MG242 Isolated from Human Vagina.
机构信息
Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon, 22212, South Korea.
MEDIOGEN, Co., Ltd., Seoul, 04157, South Korea.
出版信息
Probiotics Antimicrob Proteins. 2018 Jun;10(2):343-349. doi: 10.1007/s12602-017-9323-5.
Vulvovaginal candidiasis (VVC) is a very common infection worldwide that is mainly caused by Candida albicans. In a previous study, we showed that Lactobacillus salivarius MG242 has anti-Gardnerella vaginalis activity. In this study, we investigated the potential of using L. salivarius MG242 for biocontrol of C. albicans. In line with the results from a spot overlay assay, MG242 inhibited the growth of C. albicans by 99.99 ± 0.01% in co-culture, suggesting that L. salivarius MG242 has the potential to be developed into a probiotic formula to treat or prevent VVC. Accelerated storage tests using dehydrated live cell powder at 50, 60, and 70 °C were performed, and the results showed that immobilization with 10% skim milk effectively increased the thermal resistance of entrapped microorganisms, resulting in sevenfold longer shelf-life than the control (in PBS). Lower storage temperatures also increased the shelf-life up to 8.31 months.
外阴阴道假丝酵母菌病（VVC）是一种在全球范围内非常常见的感染，主要由白假丝酵母菌引起。在之前的研究中，我们表明唾液乳杆菌 MG242 具有抗阴道加德纳菌的活性。在本研究中，我们研究了使用唾液乳杆菌 MG242 进行生物防治白假丝酵母菌的潜力。与斑点覆盖测定的结果一致，MG242 在共培养中抑制了 99.99 ± 0.01%的白假丝酵母菌生长，这表明唾液乳杆菌 MG242 有可能被开发成一种益生菌配方，用于治疗或预防 VVC。在 50、60 和 70°C 下对脱水活菌粉末进行了加速储存试验，结果表明，用 10%脱脂乳固定化可有效提高包埋微生物的耐热性，使保质期比对照（在 PBS 中）延长了七倍。较低的储存温度也将保质期延长至 8.31 个月。
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