来自山地铁角蕨的枞酸型二萜类化合物及其抗增殖活性。

Abietane diterpenoids from Sideritis montana L. and their antiproliferative activity.

作者信息

Tóth Barbara, Kúsz Norbert, Forgo Peter, Bózsity Noémi, Zupkó István, Pinke Gyula, Hohmann Judit, Vasas Andrea

机构信息

Department of Pharmacognosy, University of Szeged, 6720 Szeged, Hungary.

Department of Pharmacodynamics and Biopharmacy, University of Szeged, 6720 Szeged, Hungary.

出版信息

Fitoterapia. 2017 Oct;122:90-94. doi: 10.1016/j.fitote.2017.08.016. Epub 2017 Aug 31.

DOI:10.1016/j.fitote.2017.08.016

PMID:28864041

Abstract

The present study aimed at the phytochemical and pharmacological investigation of Sideritis montana L. (Lamiaceae). Two new abietane diterpenes [sideritins A (1) and B (2)] were isolated from the methanol extract of the plant. Six known compounds [pomiferin E (3), 9α,13α-epi-dioxyabiet-8(14)-en-18-ol (4), paulownin (5), 6-methoxysakuranetin (6), 3-oxo-α-ionol (7) and 4-allyl-2,6-dimethoxyphenol glucoside (8)] were also obtained from the plant. The structures were determined by means of HREIMS and NMR experiments. The antiproliferative effect of the isolated compounds was investigated on human cancer cell lines (HeLa, SiHa and C33A) at 10 and 30μM concentrations, using the MTT assay. The results demonstrated that pomiferin E (3) and 6-methoxysakuranetin (6) displayed considerable activity [inhibition (%)±SEM: 46.93±2.35 on HeLa (pomiferin E), and 51.52±2.45 on C33A (6-methoxysakuranetin)] at 30μM concentration.

摘要

本研究旨在对山地岩须（唇形科）进行植物化学和药理学研究。从该植物的甲醇提取物中分离出两种新的枞烷二萜类化合物[岩须素A（1）和B（2）]。还从该植物中获得了六种已知化合物[波米菲林E（3）、9α,13α - 表二氧枞-8(14)-烯-18-醇（4）、泡桐苷（5）、6-甲氧基樱花素（6）、3-氧代-α-紫罗兰醇（7）和4-烯丙基-2,6-二甲氧基苯酚葡萄糖苷（8）]。通过高分辨电子轰击质谱（HREIMS）和核磁共振（NMR）实验确定了其结构。使用MTT法研究了分离出的化合物在10和30μM浓度下对人癌细胞系（HeLa、SiHa和C33A）的抗增殖作用。结果表明，波米菲林E（3）和6-甲氧基樱花素（6）在30μM浓度下表现出相当的活性[抑制率（%）±标准误：对HeLa细胞（波米菲林E）为46.93±2.35，对C33A细胞（6-甲氧基樱花素）为51.52±2.45]。