Shamardl Hanan A, El-Ashmony Sahar M, Kamel Hala F, Fatani Sameer H
Department of Pharmacology (HAS), Faculty of Medicine, Fayoum University, Fayoum, Egypt; Department of Pharmacology & Clinical Pharmacy (SME), Faculty of Pharmacy, Umm Al-Qura University, Makkah, Saudi Arabia.
Department of Clinical Pharmacology, Faculty of Medicine, Cairo University, Cairo, Egypt; Department of Pharmacology & Clinical Pharmacy (SME), Faculty of Pharmacy, Umm Al-Qura University, Makkah, Saudi Arabia.
Am J Med Sci. 2017 Aug;354(2):190-198. doi: 10.1016/j.amjms.2017.04.007. Epub 2017 Apr 10.
Hypertension is one of the primary modifiable risk factors for cardiovascular disease. Adequate vitamin D (vit D) levels have been shown to reduce vascular smooth muscle contraction and to increase arterial compliance, which may be beneficial in hypertension. Further, coenzyme Q10 (COQ10) through its action to lower oxidative stress has been reported to have beneficial effects on hypertension and heart failure. This study examined the possible cardiac and renal protective effects of vit D and COQ10 both separately and in combination with an angiotensin II receptor blocker, valsartan (vals) in l-NAME hypertensive rats.
Hypertension was induced in rats by l-NAME administration. Following induction of hypertension, the rats were assigned into the following 6 subgroups: an l-NAME alone group and treated groups receiving the following drugs intraperitoneally for 6 weeks; vals, vit D, COQ10 and combination of vals with either vit D or COQ10. A group of normotensive rats were used as negative controls. At the end of the treatment period, blood pressure, serum creatinine, blood urea nitrogen, lipids and serum, cardiac and renal parameters of oxidative stress were measured.
Compared to the l-NAME only group, all treatments lowered systolic, diastolic, mean arterial pressure, total cholesterol, low-density lipoprotein cholesterol, and creatinine levels as well as TNF-α and malondialdehyde. Further, the agents increased serum, cardiac and renal total antioxidant capacity. Interestingly, the combination of agents had further effects on all the parameters compared to treatment with each single agent.
The study suggests that the additive protective effects of vit D and COQ10 when used alone or concurrent with vals treatment in hypertensive rats may be due to their effects as antioxidants, anticytokines and blood pressure conservers.
高血压是心血管疾病主要的可改变风险因素之一。充足的维生素D（维D）水平已显示可减少血管平滑肌收缩并增加动脉顺应性，这可能对高血压有益。此外，据报道辅酶Q10（COQ10）通过降低氧化应激的作用对高血压和心力衰竭有有益影响。本研究在L-NAME诱导的高血压大鼠中，分别及联合血管紧张素II受体阻滞剂缬沙坦（vals），研究了维D和COQ10对心脏和肾脏可能的保护作用。
通过给予L-NAME诱导大鼠高血压。高血压诱导后，将大鼠分为以下6个亚组：单独使用L-NAME组以及腹腔注射以下药物6周的治疗组；vals、维D、COQ10以及vals与维D或COQ10的联合用药组。一组血压正常的大鼠用作阴性对照。在治疗期结束时，测量血压、血清肌酐、血尿素氮、血脂以及血清、心脏和肾脏的氧化应激参数。
与仅使用L-NAME组相比，所有治疗均降低了收缩压、舒张压、平均动脉压、总胆固醇、低密度脂蛋白胆固醇和肌酐水平以及TNF-α和丙二醛。此外，这些药物增加了血清、心脏和肾脏的总抗氧化能力。有趣的是，与单一药物治疗相比，联合用药对所有参数有进一步影响。
该研究表明，维D和COQ10单独使用或与vals联合用于高血压大鼠时的附加保护作用，可能归因于它们作为抗氧化剂、抗细胞因子和血压调节剂的作用。
