Zhao Guo-Yan, Zhao Li-Ya, Xia Zhi-Jie, Zhu Jin-Lei, Liu Di, Liu Chun-Yue, Chen Xiu-Lan, Zhang Yu-Zhong, Zhang Xi-Ying, Dai Mei-Xue
Shandong Provincial Key Laboratory of Plant Stress Research, Jinan 250014, PR China.
College of Life Science, Shandong Normal University, Jinan 250014, PR China.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1813-1819. doi: 10.1099/ijsem.0.001868. Epub 2017 Jun 12.
A Gram-stain-negative, rod-shaped bacterium, strain F01T, was isolated from leaves of Tamarix chinensis Lour. The isolate grew optimally at 30 °C, at pH 7.0 and with 5.0 % (w/v) NaCl, and showed a high tolerance to manganese, lead, nickel, ferrous ions and copper ions. The major fatty acids were C18 : 1ω7c and C16 : 0, and the predominant respiratory quinone was Q-9. Polar lipids were dominated by diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified aminoglycolipids and phospholipids. The DNA G+C content was 65.8 %. Based on multilocus phylogenetic analysis, strain F01T belonged to the genus Salinicola, with highest 16S rRNA gene sequence similarity to Salinicola peritrichatus CGMCC 1.12381T (97.7 %). The level of DNA-DNA hybridization between strain F01T and closely related Salinicola strains was well below 70 %. According to the phenotypic, genetic and chemotaxonomic data, strain F01T is considered to represent a novel species in the genus Salinicola, for which the name Salinicola tamaricis sp. nov. is proposed. The type strain is F01T (=CCTCC AB 2015304T=KCTC 42855T).
从柽柳叶片中分离出一株革兰氏阴性杆状细菌，菌株F01T。该菌株在30 °C、pH 7.0和5.0%（w/v）NaCl条件下生长最佳，对锰、铅、镍、亚铁离子和铜离子具有较高耐受性。主要脂肪酸为C18 : 1ω7c和C16 : 0，主要呼吸醌为Q-9。极性脂质以双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、未鉴定的氨基糖脂和磷脂为主。DNA G+C含量为65.8%。基于多位点系统发育分析，菌株F01T属于盐栖菌属，与周毛盐栖菌CGMCC 1.12381T的16S rRNA基因序列相似性最高（97.7%）。菌株F01T与密切相关的盐栖菌属菌株之间的DNA-DNA杂交水平远低于70%。根据表型、遗传和化学分类数据，菌株F01T被认为代表盐栖菌属中的一个新物种，为此提出名称为盐栖柽柳菌（Salinicola tamaricis sp. nov.）。模式菌株为F01T（=CCTCC AB 2015304T=KCTC 42855T）。
